Abstract
Systemic lupus erythematosus (SLE) is a systemic autoimmune and inflammatory disease with a strong genetic contribution and characterized by kinds of immune reactions. Our previous genome-wide association studies have identified IL-28RA as a susceptibility gene for SLE. In this study, we performed a quantitative reverse transcription polymerase chain reaction (RT-PCR) in 62 patients with SLE and 69 controls to investigate the different expression levels of IL-28RA in peripheral blood mononuclear cells (PBMCs) from SLE patients and healthy controls and the association between IL-28RA expression and systemic lupus erythematosus disease activity index (SLEDAI) or the variant of the single-nucleotide polymorphism (SNP) rs4649203. The expression levels of IL-28RA messenger RNA (mRNA) in SLE patients were significantly increased compared with those of healthy controls. In addition, there were also significant differences in the expression levels of IL-28RA between active (SLEDAI ≥ 6) or inactive (SLEDAI < 6) SLE groups and healthy controls. However, no correlation was observed between IL-28RA mRNA expression level and SLEDAI. There was no association between the variant of the SNP rs4649203 and IL-28RA mRNA expression levels neither. These results indicated that expression of IL-28RA mRNA may be correlated with the pathogenesis of SLE.
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Acknowledgments
We thank all the study participants and all the volunteers who have so willingly participated in this study, thus making this study possible. This work was supported by grants from the National Key Basic Research Program of China (2014CB541901, 2012CB722404, and 2011CB512103), the Research Project of Chinese Ministry of Education (No. 213018A) and the Program for New Century Excellent Talents in University (NCET-12-0600), the National Natural Science Foundation of China (81402590, 81371722, 81320108016, and 81171505), the Natural Science Fund of Anhui province (1408085MKL27) and the Youth Science Training Program of National Natural Science Foundation of China (2010kj10).
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Yu-Yan Cheng, Yu-Jun Sheng and Yan Chang contributed equally to this work.
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Cheng, YY., Sheng, YJ., Chang, Y. et al. Increased expression of IL-28RA mRNA in peripheral blood mononuclear cells from patients with systemic lupus erythematosus. Clin Rheumatol 34, 1807–1811 (2015). https://doi.org/10.1007/s10067-015-2947-5
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DOI: https://doi.org/10.1007/s10067-015-2947-5