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Uricase based methods for determination of uric acid in serum

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Abstract

The most common methods for determination of uric acid in serum are based on the use of the enzyme uricase. Uric acid is enzymatically oxidized by oxygen to produce hydrogen peroxide, allantoin, and carbon dioxide. The four most often applied uricase methods are critically compared in this review.

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Acknowledgement

This work was supported by the National Natural Science Foundation of China (No. 30672009, 30700775).

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Authors and Affiliations

  1. Unit of Biochemical Pharmacology and Protein Biotechnology, Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, College of Pharmaceutical Sciences, Chongqing Medical University, Chongqing, 400016, People’s Republic of China

    Yunsheng Zhao, Xiaoyan Yang, Wei Lu, Hong Liao & Fei Liao

  2. Department of Clinic Laboratory, The First Hospital of Qinhuangdao, Qinhuangdao, 066000, Hebei, People’s Republic of China

    Yunsheng Zhao

  3. Chongqing Key Laboratory of Biochemistry and Molecular Pharmacology, College of Pharmaceutical Sciences, Chongqing Medical University, Chongqing, 400016, People’s Republic of China

    Fei Liao

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  1. Yunsheng Zhao
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  2. Xiaoyan Yang
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  3. Wei Lu
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  4. Hong Liao
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  5. Fei Liao
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Correspondence to Fei Liao.

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Zhao, Y., Yang, X., Lu, W. et al. Uricase based methods for determination of uric acid in serum. Microchim Acta 164, 1–6 (2009). https://doi.org/10.1007/s00604-008-0044-z

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  • DOI: https://doi.org/10.1007/s00604-008-0044-z

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