Peritoneal closure reduces port site metastases

Abstract

Background: The arm of this study was to assess the role of peritoneal closure in the prevention of port site metastasis after carbon dioxide (CO₂) CO2 pneumoperitoneum. Methods: We developed a xenograft ovarian cancer model by intraperitoneal injection of 27 106 IGR-OV1 line cells in nude rats Seven days after the innoculation, the animals underwent a CO₂ pneumoperitoneum. At the end of the procedure, port sites were randomly closed either with suture of peritoneum (n = 14, group A) or without suture of peritoneum (n = 12, group B). The rats were killed 7 days after surgery and their port site scars were resected. Tumor implantation was assessed by a pathologist who was blinded to the type of wound closure. Results: The animals in group B were significantly more likely to have at least one port site metastasis frequent (seven of 12, or 58.3%) than those in group A (two of 14, or (14.3%) (p = 0.037). Port sites with metastases were seen more frequently in group B (eight of 24, or (33.3%) than in group A (three of 28, or 10.7%) (p = 0.046). Conclusions: Our results shows that peritoneum closure decreases the risk of port site metastasis.