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The effects of the stem cell on ciliary regeneration of injured rabbit sinonasal epithelium

  • Rhinology
  • Published:
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Abstract

Defects in mucosal healing after sinonasal surgery cause infection, scar formation causing obstruction, relapse of the disease within a shorter period and revision surgery. The present study aimed to create a functional ciliated epithelium using a stem cell and stem cell sheet of adipose tissue origin and to show such regeneration ultra-structurally on experimentally injured rabbit nasal epithelium. This was an experimental animal study and basic research. A total of 18 white New Zealand rabbits were divided into three groups. The medial wall of the maxillary sinus of the subjects was peeled off bilaterally. No additional procedure was applied to the subjects in Group 1. In Group 2, adipose tissue-derived mesenchymal stem cell was implanted on the wound edges of the subjects. In Group 3, a stem cell sheet of three layers was laid onto the defect area. All subjects were killed after 3 weeks. The presence of the stem cell stained with bromo-deoxyuridine was assessed with a light microscope, whereas cilia density, ciliated orientation and cilia structure were evaluated with a scanning electron microscope. Ciliary densities in Group 2 and Group 3 were statistically superior compared to the control group (p < 0.001, p = 0.007). Cilia morphology in Group 2 and Group 3 was also better than the control group (p < 0.01, p = 0.048). Ciliary orientation in Group 2 was scored highest (p < 0.01). The ratio of BrDu-stained cells was observed to be 27% in Group 3 and 8% in Group 2. Sub-epithelial recovery was observed to be better in Group 3. Adipose tissue-derived mesenchymal stem cell increased the healing of the injured maxillary sinus mucosa of the rabbits in terms of cilia presence, density and morphology regardless of the implementation technique.

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Authors and Affiliations

  1. Department of Otorhinolaryngology and Head and Neck Surgery, Dışkapı Yıldırım Beyazıt Research and Training Hospital, Ministiry of Health, Ankara, Turkey

    Ali Kavuzlu, Emel Çadallı Tatar, Tuğba Karagöz & Ömer Bayır

  2. Department of Stem Cell and Genetic Diagnosis Center, Dışkapı Yıldırım Beyazıt Research and Training Hospital, Ministiry of Health, Ankara, Turkey

    Ferda Alpaslan Pınarlı

  3. Department of Anatomy, Faculty of Medicine, Hacettepe University, Ankara, Turkey

    İlkan Tatar

  4. Department of Otolaryngology and Head and Neck Surgery, Faculty of Medicine, Yıldırım Beyazıt University, Ankara, Turkey

    Mehmet Hakan Korkmaz

Authors
  1. Ali Kavuzlu
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  2. Emel Çadallı Tatar
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  3. Tuğba Karagöz
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  4. Ferda Alpaslan Pınarlı
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  5. İlkan Tatar
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  7. Mehmet Hakan Korkmaz
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Correspondence to Ali Kavuzlu.

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Funding

The study was financially supported by a Grant from Dışkapı Yıldırım Beyazıt Training and Research Hospital Scientific Research Support Committee (47/3/2014).

Conflict of interest

The authors declare that they have no conflict of interest.

Ethical approval

All applicable international, national, and/or institutional guidelines for the care and use of animals were followed.

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Kavuzlu, A., Tatar, E.Ç., Karagöz, T. et al. The effects of the stem cell on ciliary regeneration of injured rabbit sinonasal epithelium. Eur Arch Otorhinolaryngol 274, 3057–3064 (2017). https://doi.org/10.1007/s00405-017-4595-7

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  • DOI: https://doi.org/10.1007/s00405-017-4595-7

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