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Chronic ACE inhibitor treatment increases angiotensin type 1 receptor binding in vivo in the dog kidney

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Abstract

Purpose

PET imaging has been recently introduced for investigating the type 1 angiotensin II receptor (AT1R) in vivo. The goal of the present study was to investigate the effects of acute and chronic exposure to angiotensin converting enzyme inhibitors (ACEI) on the AT1R in the dog kidney.

Methods

Animals were imaged at baseline, after acute intravenous ACEI treatment and after a chronic 2-week exposure to an oral ACEI. Control animals were imaged at identical time points in the absence of ACEI treatment.

Results

In vivo AT1R binding expressed by K i was increased in the renal cortex by chronic ACEI treatment (p < 0.05). In vitro measurements of AT1R density (B max) also revealed significant increases in AT1R in isolated glomeruli (p < 0.05). Plasma renin activity was increased, but angiotensin II (Ang II) and the Ang II/Ang I ratio showed a weak correlation with chronic ACEI treatment, consistent with an Ang II escape phenomenon.

Conclusion

This study reveals, for the first time, that chronic ACEI treatment increases AT1R binding in vivo in the dog renal cortex.

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Acknowledgment

We thank Paige Finley for the help in preparation, monitoring, and postprocedural care of the experimental animals. We also appreciate the contributions of the personnel of the PET center (David Clough, Karen Edmonds, Michael Hans, Hayden Ravert and Robert Smoot) during the organization and performance of the PET studies. This work was supported by the NIH grant #RO1 DK050183.

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Authors and Affiliations

  1. Departments of Radiology and Surgery, Johns Hopkins University, Baltimore, MD, USA

    Tamas G. Zober, Phillip R. Brown, Esen Seckin, William B. Mathews & Zsolt Szabo

  2. Department of Pathophysiology, Semmelweis University, Budapest, Hungary

    Tamas G. Zober

  3. Department of Medicine, Georgetown University, Washington, DC, USA

    Maria Elena Fabucci, Wei Zheng & Kathryn Sandberg

  4. Division of Nuclear Medicine, Johns Hopkins Outpatient Center, 601 N. Caroline Street, Rm. JHOC 3233, Baltimore, MD, 21287-0817, USA

    Zsolt Szabo

Authors
  1. Tamas G. Zober
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  2. Maria Elena Fabucci
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  3. Wei Zheng
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  4. Phillip R. Brown
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  5. Esen Seckin
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  6. William B. Mathews
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  7. Kathryn Sandberg
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  8. Zsolt Szabo
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Correspondence to Zsolt Szabo.

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Zober, T.G., Fabucci, M.E., Zheng, W. et al. Chronic ACE inhibitor treatment increases angiotensin type 1 receptor binding in vivo in the dog kidney. Eur J Nucl Med Mol Imaging 35, 1109–1116 (2008). https://doi.org/10.1007/s00259-007-0667-z

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  • DOI: https://doi.org/10.1007/s00259-007-0667-z

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