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Influences of Flavonoids on Erythrocyte Membrane and Metabolic Implication Through Anionic Exchange Modulation

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Abstract

The antioxidative activity of some natural flavonoids was analyzed against the stable free radical 2,2-diphenyl-1-picryhydrazyl. The results indicate that the scavenging power of the tested flavonols is higher than that of the synthetic antioxidants butylated hydroxyanisole and butylated hydroxytoluene; instead, the flavanones show little activity, as indicated by efficient concentration (EC50) values. Flavonoid autoxidation and interaction with Fe2+ and hydrogen peroxide were tested using erythrocyte membranes as a model. The results show that some compounds, like hesperetin, evidence a pro-oxidant activity higher than the ascorbic acid/iron reference system. The compounds with strong oxidative capability do not only influence cellular redox balance but also activate caspase-3, producing lactate dehydrogenase release and enhancing anionic exchange at the level of band 3 protein.

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Authors and Affiliations

  1. Organic and Biological Chemistry Department, University of Messina, Messina, Italy

    Davide Barreca, Giuseppina Laganà, Ester Tellone, Silvana Ficarra, Ugo Leuzzi, Antonio Galtieri & Ersilia Bellocco

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  1. Davide Barreca
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  2. Giuseppina Laganà
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  3. Ester Tellone
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  4. Silvana Ficarra
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  5. Ugo Leuzzi
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  6. Antonio Galtieri
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  7. Ersilia Bellocco
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Correspondence to Antonio Galtieri.

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Barreca, D., Laganà, G., Tellone, E. et al. Influences of Flavonoids on Erythrocyte Membrane and Metabolic Implication Through Anionic Exchange Modulation. J Membrane Biol 230, 163–171 (2009). https://doi.org/10.1007/s00232-009-9197-x

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  • DOI: https://doi.org/10.1007/s00232-009-9197-x

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