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Molecular mapping of stripe rust resistance gene YrCH42 in Chinese wheat cultivar Chuanmai 42 and its allelism with Yr24 and Yr26

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Abstract

Stripe rust, caused by Puccinia striiformis f. sp. tritici (PST), is one of the most devastating diseases in common wheat (Triticum aestivum L.) worldwide. The objectives of this study were to map a stripe rust resistance gene in Chinese wheat cultivar Chuanmai 42 using molecular markers and to investigate its allelism with Yr24 and Yr26. A total of 787 F2 plants and 186 F3 lines derived from a cross between resistant cultivar Chuanmai 42 and susceptible line Taichung 29 were used for resistance gene tagging. Also 197 F2 plants from the cross Chuanmai 42×Yr24/3*Avocet S and 726 F2 plants from Chuanmai 42×Yr26/3*Avocet S were employed for allelic test of the resistance genes. In all, 819 pairs of wheat SSR primers were used to test the two parents, as well as resistant and susceptible bulks. Subsequently, nine polymorphic markers were employed for genotyping the F2 and F3 populations. Results indicated that the stripe rust resistance in Chuanmai 42 was conferred by a single dominant gene, temporarily designated YrCH42, located close to the centromere of chromosome 1B and flanked by nine SSR markers Xwmc626, Xgwm273, Xgwm11, Xgwm18, Xbarc137, Xbarc187, Xgwm498, Xbarc240 and Xwmc216. The resistance gene was closely linked to Xgwm498 and Xbarc187 with genetic distances of 1.6 and 2.3 cM, respectively. The seedling tests with 26 PST isolates and allelic tests indicated that YrCH42, Yr24 and Yr26 are likely to be the same gene.

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Acknowledgements

The authors are grateful to Prof. R.A. McIntosh for his critical review of this manuscript. They are also grateful to Dr. C.R. Wellings, Plant Breeding Institute, University of Sydney, Australia, for providing a set of near-isogenic lines with different stripe rust resistance genes. This project was funded by National 863 program (2003AA207090) and National Natural Science Foundation of China (30471083 and 30220140636).

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Authors and Affiliations

  1. Institute of Crop Science/National Wheat Improvement Center, Chinese Academy of Agricultural Sciences (CAAS), Zhongguancun South Street 12, 100081, Beijing, China

    G. Q. Li, Z. F. Li, Z. H. He & X. C. Xia

  2. Crop Research Institute, Sichuan Academy of Agricultural Sciences, Jingjusi Street 20, 610066, Chengdu, Sichuan, China

    W. Y. Yang & Y. Zhang

  3. Faculty of Agronomy, Xinjiang Agricultural University, Nanchang Road 42, 830052, Urumqi, Xinjiang, China

    G. Q. Li & Y. Y. Qu

  4. International Maize and Wheat Improvement Center (CIMMYT) China Office, c/o CAAS, Zhongguancun South Street 12, 100081, Beijing, China

    Z. H. He

  5. Institute of Plant Protection, Chinese Academy of Agricultural Sciences (CAAS), Yuanmingyuan West Road 2, 100094, Beijing, China

    S. C. Xu

  6. International Maize and Wheat Improvement Center (CIMMYT), Apdo. Postal 6-641, 06600, Mexico DF, Mexico

    R. P. Singh

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  1. G. Q. Li
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  2. Z. F. Li
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Correspondence to Z. H. He or X. C. Xia.

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Communicated by B. Friebe

G.Q. Li and Z.F. Li contributed equally to the work.

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Li, G.Q., Li, Z.F., Yang, W.Y. et al. Molecular mapping of stripe rust resistance gene YrCH42 in Chinese wheat cultivar Chuanmai 42 and its allelism with Yr24 and Yr26 . Theor Appl Genet 112, 1434–1440 (2006). https://doi.org/10.1007/s00122-006-0245-y

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