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Fertility restorer locus Rf1 of sorghum (Sorghum bicolor L.) encodes a pentatricopeptide repeat protein not present in the colinear region of rice chromosome 12

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An Erratum to this article was published on 29 November 2005

An Erratum to this article was published on 29 November 2005

Abstract

With an aim to clone the sorghum fertility restorer gene Rf1, a high-resolution genetic and physical map of the locus was constructed. The Rf1 locus was resolved to a 32-kb region spanning four open reading frames: a plasma membrane Ca2+-ATPase, a cyclin D-1, an unknown protein, and a pentatricopeptide repeat (PPR13) gene family member. An ~19-kb region spanning the cyclin D-1 and unknown protein genes was completely conserved between sterile and fertile plants as was the sequence spanning the coding region of the Ca2+-ATPase. In contrast, 19 sequence polymorphisms were located in an ~7-kb region spanning PPR13, and all markers cosegregated with the fertility restoration phenotype. PPR13 was predicted to encode a mitochondrial-targeted protein containing a single exon with 14 PPR repeats, and the protein is classified as an E-type PPR subfamily member. To permit sequence-based comparison of the sorghum and rice genomes in the Rf1 region, 0.53 Mb of sorghum chromosome 8 was sequenced and compared to the colinear region of rice chromosome 12. Genome comparison revealed a mosaic pattern of colinearity with an ~275-kb gene-poor region with little gene conservation and an adjacent, ~245-kb gene-rice region that is more highly conserved between rice and sorghum. Despite being located in a region of high gene conservation, sorghum PPR13 was not located in a colinear position on rice chromosome 12. The present results suggest that sorghum PPR13 represents a potential candidate for the sorghum Rf1 gene, and its presence in the sorghum genome indicates a single gene transposition event subsequent to the divergence of rice and sorghum ancestors.

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Acknowledgements

The authors wish to thank Ms. Julie McCollum (USDA–ARS) and Tracie Miner (Washington University) for expert technical assistance. Special thanks to Professor Ian Small (Unité de Recherches en Gènomique Végétale INRA CNRS, Evry, France) for critical guidance and thoughtful insight concerning PPR genes. The research is supported by the United States Department of Agriculture’s Agricultural Research Service (R.R.K.). This work was also supported in part by National Science Foundation Plant Genome Grant DBI-0077713 (J.E.M. and P.E.K) and DBI-0321578 (P.E.K., R.R.K. and J.E.M.).

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Authors and Affiliations

  1. Southern Plains Agricultural Research Center, USDA–ARS, College Station, TX, 77845, USA

    R. R. Klein & K. F. Schertz

  2. Department of Horticulture and Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, TX, 77843-2123, USA

    P. E. Klein

  3. Department of Biochemistry and Biophysics and Institute for Plant Genomics and Biotechnology, Texas A&M University, College Station, TX, 77843-2123, USA

    J. E. Mullet

  4. Washington University Genome Sequencing Center, Washington University School of Medicine, St. Louis, MO, 63108, USA

    P. Minx

  5. Department of Soil and Crop Sciences, Texas A&M University, College Station, TX, 77843-2474, USA

    W. L. Rooney

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  1. R. R. Klein
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Correspondence to R. R. Klein.

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Communicated by E. Guiderdoni

An erratum to this article can be found at http://dx.doi.org/10.1007/s00122-005-0134-9

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Klein, R.R., Klein, P.E., Mullet, J.E. et al. Fertility restorer locus Rf1 of sorghum (Sorghum bicolor L.) encodes a pentatricopeptide repeat protein not present in the colinear region of rice chromosome 12. Theor Appl Genet 111, 994–1012 (2005). https://doi.org/10.1007/s00122-005-2011-y

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