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A ‘Chinese Spring’ wheat (Triticum aestivum L.) bacterial artificial chromosome library and its use in the isolation of SSR markers for targeted genome regions

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Abstract

A bacterial artificial chromosome (BAC) library was constructed from the bread wheat (Triticum aestivum L.) genotype ‘Chinese Spring’ (‘CS’). The library consists of 395,136 clones with an estimated average insert size of 157 kb. This library provides an estimated 3.4-fold genome coverage for this hexaploid species. The genome coverage was confirmed by RFLP analysis of single-copy RFLP clones. The CS BAC library was used to develop simple sequence repeat (SSR) markers for targeted genome regions using five sequence-tagged-site (STS) markers designed from the chromosome arm of 3BS. The SSR markers for the targeted genome region were successfully obtained. However, similar numbers of new SSR markers were also generated for the other two homoeologous group 3 chromosomes. This data suggests that BAC clones belonging to all three chromosomes of homoeologous group 3 were isolated using the five STS primers. The potential impacts of these results on marker isolation in wheat and on library screening in general are discussed.

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Acknowledgements

The authors are grateful to Tom Magner and Miki Miyagi for their excellent technical assistance, to Prof. M.D. Gale (John Innes Centre, UK) for constructive discussions and Dr. Evans Lagudah (CSIRO Plant Industry) for the ‘CS’ aneuploid lines. This project was partially supported by the Grains Research and Development Corporation (grant number ET7/CSP00034) of Australia.

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Authors and Affiliations

  1. CSIRO Plant Industry, 306 Carmody Road, St Lucia, Queensland, 4067, Australia

    B. Shen, D.M. Wang, C.L. McIntyre & C.J. Liu

  2. College of life science, Hebei Agricultural University, Baoding, Hebei, China

    D.M. Wang

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  1. B. Shen
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  2. D.M. Wang
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  3. C.L. McIntyre
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  4. C.J. Liu
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Correspondence to C.J. Liu.

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Communicated by J. W. Snape

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Shen, B., Wang, D., McIntyre, C. et al. A ‘Chinese Spring’ wheat (Triticum aestivum L.) bacterial artificial chromosome library and its use in the isolation of SSR markers for targeted genome regions. Theor Appl Genet 111, 1489–1494 (2005). https://doi.org/10.1007/s00122-005-0077-1

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  • DOI: https://doi.org/10.1007/s00122-005-0077-1

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