Abstract.
Factor XIII subunit A of blood coagulation (FXIII-A) is known to be synthesized but not secreted by the monocyte/macrophage cell line. On the basis of its intracellular localization and substrate profile, FXIII-A is thought to be involved in certain intracellular processes. Our present study was designed to monitor the changes in FXIII-A gene expression and protein production in long-term culture of human monocytes during their differentiation into macrophages in the presence of activating agents (interleukin-4, interferon-γ, Mycobacterium bovis BCG) inducing classical and alternative activation pathways. By using quantitative RT-PCR and fluorescent image analysis at the single-cell level we demonstrated that the expression of FXIII-A both at the mRNA as well as at the protein level is inversely regulated during the two activation programmes. Here we conclude that FXIII-A expression is an intracellular marker for alternatively activated macrophages, while its absence in monocyte-derived macrophages indicates their classically activated state.
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Received 2 June 2005; received after revision 12 July 2005; accepted 22 July 2005
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Törőcsik, D., Bárdos, H., Nagy, L. et al. Identification of factor XIII-A as a marker of alternative macrophage activation. Cell. Mol. Life Sci. 62, 2132–2139 (2005). https://doi.org/10.1007/s00018-005-5242-9
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DOI: https://doi.org/10.1007/s00018-005-5242-9