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High-speed determination of aesculin and aesculetin inCortex fraxini by micellar electrokinetic chromatography

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  • Micellar Elexctrokinetic Chromatography
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Summary

A new rapid micellar electrokinetic chromatographic (MEKC) method has been developed for the determination of two kinds of coumarin (aesculin and aesculetin) inCortex fraxini. After a series of optimization experiments, 100 mM boric acid (pH 9.5), 50 mM sodium dodecyl sulfate (SDS), 30 kV applied voltage and 35°C temperature were selected. The contents of aesculin and aesculetin inCortex fraxini were successfully determined within 6 min by this method with quercetin as internal standard. The relative standard deviations (RSD) of the method are 1,22% for aesculin and 1.02% for aesculetin on the basis of peak areas. The detection limits (3S/N) are 25 μg mL−1 for aesculin and 20 μg mL−1 for aesculetin. Calibration curves are linear in the concentration ranges of 136–1020 μg mL−1 for aesculin (r=0.9969) and 126–945 μg mL−1 for aesculetin (r=0.9993), and the recoveries are 98.5% for aesculin and 98.6% for aesculetin.

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Bo, T., Liu, H. & Li, K.A. High-speed determination of aesculin and aesculetin inCortex fraxini by micellar electrokinetic chromatography. Chromatographia 55, 621–624 (2002). https://doi.org/10.1007/BF02492912

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  • DOI: https://doi.org/10.1007/BF02492912

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