Abstract
Completely damaged wheat grains, unfit for human consumption, were fermented to H2 by Bacillus licheniformis strain JK1. Batch-culture fermentation of wheat slurries [6% (w/v) total solids and 5.8% (w/v) organic solids (OS)] evolved 225, 205 and 203 l of biogas-H, a mixture of H2, CO2 and H2S, per kg OS at pH 6, 7 and 8, respectively. H2 constituted 25% to 41% of the total biogas-H evolved. In single-stage continuous culture, H2 generated/kg OS reduced was 70 l at pH 6 and 74 l at pH 7 and 8.
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References
Archer, D.B. & Thompson, L.A. 1987 Energy production through the treatment of wastes by micro-organisms. Journal of Applied Bacteriology, Symposium Supplement 59S–70S.
Bollinger, R., Zurrer, M. & Bachofen, R. 1985 Photoproduction of molecular hydrogen from waste water of a sugar refinery by photosynthetic bacteria. Applied Microbiology and Biotechnology 23, 147–151.
Cox, K.E. & Williamson, K.D. 1977 Hydrogen, its Technology and Implications. Cleveland: CRC Press.
El-Shinnawi, M.M., El-Houssini, M., Aboel-Naga, S.A. & Fahmy, S. 1990 Chemical changes during a two-stage disgestion technique for biogas production from combinations of organic wastes. Resources, Conservation and Recycling 3, 217–230.
Goodwin, S., Conrad, R. & Zeikus, J.G. 1988 Influence of pH on microbial hydrogen metabolism in diverse sedimentary ecosystems. Applied and Environmental Microbiology 54, 590–593.
Jain, S.R. 1992 Isolation and characterization of hydrogen producing bacteria and their potential to transform different organic substrates to hydrogen. Ph.D. Thesis. University of Delhi, India.
Joshi, A.P., Ramachandran, P.G. & Tulsani, N.B. 1986 Bioconversion of apple waste into hydrogen and methane. Patent 165976.
Kalia, V.C., Kumar, A., Jain, S.R. & Joshi, A.P. 1992 Methanogenesis of dumping wheat grains and recycling of the effluent. Resources, Conservation and Recycling 6, 161–166.
Kalia, V.C., Kumar, A. & Joshi, A.P. 1993 Damaged wheat grains: a source for hydrogen and methane. In Proceedings of the International Recycling Congress '93, Vol. 4, Geneva. in press.
Khoshoo, T.N. 1991 Energy from plants: problems and prospects. In Environmental Concerns and Strategies, Khoshoo, T.N. eds pp. 257–372. New Delhi: Ashish.
Kosaric, N. & Lyng, R.P. 1988 Microbial production of hydrogen. In Biotechnology, Vol. 6B, eds Rehm, H.J. & Reed, G. pp. 101–134. Weinheim: Vett.
Koster, I.W. 1984 Liquefaction and acidogenesis of tomatoes in anaerobic two phase solid-waste treatment system. Agricultural Wastes 11, 241–252.
Mah, R.A. 1982 Methanogenesis and methanogenic partnerships. Philosophical Transactions of the Royal Society, Series B 297, 599–616.
Mudrack, K., Sahm, H. & Sittig, W. 1987 Environmental biotechnology: purification of effluents by anaerobic process. In Fundamentals of Biotechnology, eds Preve, P., Paust, U., Sittig, W. & Sukatsch, D.A. pp. 633–660. Weinheim: VCH.
Ormerod, J.G. 1983 The carbon cycle in aquatic ecosystems. In Microbes in their Natural Environment, eds Slater, J.H., Wittenbury, R. & Wimpenny, J.W.T. pp. 463–482. Cambridge: Cambridge University Press.
Roychowdhury, S., Cox, D. & Levandowsky, M. 1988 Production of hydrogen by microbial fermentation. International Journal of Hydrogen Energy 13, 407–410.
Salih, F.M. 1989 Improvement of hydrogen photoproduction from E. coli pre-treated cheese whey. International Journal of Hydrogen Energy 14, 661–663.
Tanisho, S., Suzuki, Y. & Wakao, N. 1987 Fermentative hydrogen evolution by Enterobacter aerogenes strain E.82005. International Journal of Hydrogen Energy 12, 623–627.
Thauer, R. 1977 Limitation of microbial H2 formation via fermentation. In Microbial Energy Conversion, eds Schlegel, H.G. & Barnea, J. pp 201–204. New York: Pergamon Press.
Ueki, K., Ueki, A. & Simogoh, Y. 1988 Terminal steps in the anaerobic digestion of municipal sewage sludge: effects of inhibitors of methanogenesis and sulfate reduction. Journal of General and Applied Microbiology 34, 425–432.
Verrier, D., Roy, F. & Albagnac, G. 1987 Two-phase methanization of solid vegetable wastes. Biological Wastes 22, 163–167.
Wolin, M. & Miller, T. 1980 Molybdate and sulfate inhibit H2 and increase formate production from glucose by Ruminococcus albus. Archives of Microbiology 124, 137.
Zurrer, H. & Bachofen, R. 1982 Aspects of growth and hydrogen production of the photosynthetic bacterium Rhodospirillum rubrum in continuous culture. Biomass 2, 165–174.
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V.C. Kalia, S.R. Jain, and A. Kumar are and A.P. Joshi was with the Centre for Biochemical Technology, Mall Road, University Campus, Delhi-110007, India; A.P. Joshi is now with the Chemical Engineering Division, National Chemical Laboratory, Pune-410008, India.
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Kalia, V.C., Jain, S.R., Kumar, A. et al. Frementation of biowaste to H2 by Bacillus licheniformis . World Journal of Microbiology & Biotechnology 10, 224–227 (1994). https://doi.org/10.1007/BF00360893
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DOI: https://doi.org/10.1007/BF00360893