Abstract
Toxins, enzymes, and biologically active peptides are the main components of snake venoms from the genusBothrops. Following the venom inoculation, the local effects are hemorrhage, edema, and myonecrosis.
Nineteen different species of BrazilianBothrops were screened for protein content andl-amino acid oxidase activity.B. cotiara, formerly found in the South of Brazil, is now threatened with extinction. Its venom contains a highly hemorrhagic fraction and, as expected from the deep yellow color of the corresponding lyophilized powder, a highl-amino acid oxidase (LAO) activity was also characterized. Flavin adenine dinucleotide (FAD) is its associate coenzyme.B. cotiara venom LAO catalyzed the oxidative deamination of severall-amino acids, and the best substrates were methionine, leucine, tryptophan, and phenylalanine, hence, its potential application for the use in biosensors for aspartame determination and for the removal of amino acids from plasma. High levels for LAO were also found in other species thanB. cotiara. In addition, the technique of isoelectric focusing (IEF) was employed as a powerful tool to study the iso-or multienzyme distribution for LAO activity in theB. cotiara snake venom.
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An erratum to this article is available at http://dx.doi.org/10.1007/BF02786867.
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Pessatti, M.L., Fontana, J.D., Furtado, M.F.D. et al. Screening ofBothrops snake venoms forl-amino acid oxidase activity. Appl Biochem Biotechnol 51, 197–210 (1995). https://doi.org/10.1007/BF02933424
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DOI: https://doi.org/10.1007/BF02933424