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Plant-virus-based vectors for gene transfer will be of limited use because of the high error frequency during viral RNA synthesis

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Summary

The error frequency during the RNA replication of alfalfa mosaic virus (AMV) was calculated to be significantly higher than 10−5. It may be expected that RNA synthesis in general will have low fidelity compared to DNA synthesis. The low fidelity of RNA replication will severely restrict the usefulness of vectors for genetic engineering which are based on RNA viruses, viroids or DNA viruses which are replicated via an RNA intermediate (e.g. caulimoviruses). Spontaneous mutants selected by host shift were found to be much less stable than UV-induced mutants. This difference points to variations in fidelity during RNA synthesis, probably due to the local sequence of the template.

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References

  1. Alblas F, Bol JF: Factors influencing the infection of cowpea mesophyll protoplasts by alfalfa mosaic virus. J Gen Virol 36:175–185, 1977.

    Google Scholar 

  2. Barker RF, Jarvis NP, Thompson DV, Loesch-Fries LS, Hall TC: Complete nucleotide sequence of alfalfa mosaic virus RNA 3. Nucl Acids Res 11:2881–2891, 1983.

    CAS  PubMed  Google Scholar 

  3. Bol JF, Van Vloten-Doting L, Jaspars EMJ: A functional equivalence of top componenta RNA and coat protein in the initiation of infection by alfalfa mosaic virus. Virology 46:73–85, 1971.

    Article  CAS  PubMed  Google Scholar 

  4. Buck KW, Coutts RMA: The potential of geminiviruses in the construction of plant host vector systems. Plant Mol Biol 2:351–357, 1983.

    Article  Google Scholar 

  5. Cornelissen BJC, Brederode FTh, Veeneman GH, Van Boom JH, Bol JF: Complete nucleotide sequence of alfalfa mosaic virus RNA 2. Nucl Acids Res 11:3019–3025, 1983.

    CAS  PubMed  Google Scholar 

  6. Cornelissen BJC, Brederode FTh, Moormann RJM, Bol JF: Complete nucleotide sequence of alfalfa mosaic virus RNA 1. Nucl Acids Res 11:1253–1265, 1983.

    CAS  PubMed  Google Scholar 

  7. Dingjan-Versteegh A, Van Vloten-Doting L, Jaspars EMJ: Confirmation of the construction of alfalfa mosaic virus hybrid genomes by backcross experiments. Virology 59:328–330, 1974b.

    Article  CAS  PubMed  Google Scholar 

  8. Dingjan-Versteegh A, Van Vloten-Doting L, Jaspars EMJ: Alfalfa mosaic virus hybrids constructed by exchanging nucleoprotein components. Virology 49: 716–722, 1972.

    Article  CAS  PubMed  Google Scholar 

  9. Domingo E, Sabo D, Taniguchi T, Weissmann C: Nucleotide sequence heterogeneity of an RNA phage population. Cell 13:735–744, 1978.

    Article  CAS  PubMed  Google Scholar 

  10. Donis-Keller H, Browning KS, Clark JM: Sequence heterogeneity in Satellite Tobacco Necrosisvirus RNA. Virology 110:43–54, 1981.

    Article  CAS  Google Scholar 

  11. Garcia-Arenal F, Palukaitis P, Zaitlin M: Strains and mutants of tobacco mosaic virus are both found in virus derived from single-lesion-passaged inoculum. Virology 132:131–137, 1984.

    Article  CAS  Google Scholar 

  12. Goelet P, Lomonossoff GP, Butler PJG, Akain ME, Gait MJ, Karn J: Nucleotide sequence of tobacco mosaic virus RNA. PNAS 79:5818–5822, 1982.

    CAS  PubMed  Google Scholar 

  13. Holland J, Spindler K, Horodyski F, Grabau E, Nichol S, Van Der Pol J: Rapid evolution of RNA genomes. Science 215:1577–1585, 1982.

    CAS  PubMed  Google Scholar 

  14. Howell S: Plant Molecular Vehicles: Potential vectors for introducing foreign DNA into plants. Ann Rev of Plant Physiology 33:609–650, 1982.

    Article  CAS  Google Scholar 

  15. Hull R, Covey SN: Does cauliflower mosaic virus replicate by reverse transcription. Trends Biochem Sci 8:119–121, 1983.

    Article  CAS  Google Scholar 

  16. Hull R, Davies JW: Genetic engineering with plant viruses, and their potential as vectors. Adv Virus Res 28:1–35, 1983.

    CAS  PubMed  Google Scholar 

  17. King AMQ, McCahon D, Slade WR, Newman JWI: Recombination in RNA. Cell 29:921–928, 1982.

    Article  CAS  PubMed  Google Scholar 

  18. Kunkel LO: Amm Assoc Adv Sci Publ 12:22, 1940.

    Google Scholar 

  19. Matthews REF: Plant virology, 2nd edition. Academic Press, New York, 1981.

    Google Scholar 

  20. Nassuth A, Bol JF: Altered balance of the synthesis of plus-and minus-strand RNAs induced by RNA 1 and RNA 2 of alfalfa mosaic virus in the absence of RNA 3. Virology 124:75–85, 1982.

    Article  Google Scholar 

  21. Nassuth A, Ten Bruggencate G, Bol JF: Time course of alfalfa mosaic virus RNA and coat protein synthesis in cowpea protoplasts. Virology 125:75–84, 1983.

    Article  CAS  Google Scholar 

  22. Pfeiffer P, Hohn T: Involvement of reverse transcription in the replication of cauliflower mosaic virus: a detailed model and test of some aspects. Cell 33:781–789, 1983.

    Article  CAS  PubMed  Google Scholar 

  23. Reanney D: Genetic noise in evolution. Nature 307:318–319, 1984.

    Article  CAS  PubMed  Google Scholar 

  24. Roosien J, Van Vloten-Doting L: Complementation and interference of UV induced Mts mutants of alfalfa mosaic virus. J Gen Virol 63:189–198, 1982.

    Google Scholar 

  25. Takebe J, Otsuki Y: Infection of tobacco mesophyll protoplasts by tobacco mosaic virus. Proc Natl Acad Sci USA 64:843–848, 1969.

    CAS  PubMed  Google Scholar 

  26. Van Vloten-Doting L: Advantages of multipartite genomes of single-stranded RNA plant viruses in nature, for research and for genetic engineering. Plant Mol Biol Reporter 1:55–60, 1983.

    Google Scholar 

  27. Van Vloten-Doting L, Kruseman J, Jaspars EMJ: The biological function and mutual dependence of bottom component and top componenta of alfalfa mosaic virus. Virology 34:728–737, 1968.

    Article  PubMed  Google Scholar 

  28. Van Vloten-Doting L, Hasrat JA, Oosterwijk E, Van 't Sant P, Schoen MA, Roosien J: Description and complementation analysis of 13 temperature-sensitive mutants of alfalfa mosaic virus. J Gen Virol 46:415–426, 1980.

    Google Scholar 

  29. Yarwood CE: Host passage effects with plant viruses. Advances in Virus Research 25:169–187, 1979.

    Article  CAS  PubMed  Google Scholar 

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van Vloten-Doting, L., Bol, JF. & Cornelissen, B. Plant-virus-based vectors for gene transfer will be of limited use because of the high error frequency during viral RNA synthesis. Plant Mol Biol 4, 323–326 (1985). https://doi.org/10.1007/BF02418253

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