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Hydroxyl radical-scavenging property of indomethacin

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Abstract

The ability of indomethacin to scavenge hydroxyl radical (.OH) using high pressure liquid chromatography (HPLC) was investigated. .OH radical was generated by photolysis of H2O2 (1.5–10 mmoles/L) with UV light and was trapped with salicyclic acid (500 nmoles). H2O2 produced .OH in a concentration-dependent manner as estimated by .OH adduct products 2,3- and 2,5-dihydroxybenzoic acid (DHBA). Indomethacin in increasing concentrations (5–600 μmoles/L) produced increasing inhibition of generation of 2,3-DHBA (7–67%) and of 2,5-DHBA (7–77%). The results indicate that indomethacin scavenges .OH in a concentration-dependent manner.

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Authors and Affiliations

  1. Department of Physiology, College of Medicine, University of Saskatchewan, S7N 0WO, Saskatoon, Saskatchewan, Canada

    Kailash Prasad & Victor A. Laxdal

  2. Department of Pathology, College of Medicine, University of Saskatchewan, S7N 0WO, Saskatoon, Saskatchewan, Canada

    Kailash Prasad & Victor A. Laxdal

  3. Royal University Hospital, S7N 0WO, Saskatoon, Saskatchewan, Canada

    Kailash Prasad & Victor A. Laxdal

Authors
  1. Kailash Prasad
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  2. Victor A. Laxdal
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Prasad, K., Laxdal, V.A. Hydroxyl radical-scavenging property of indomethacin. Mol Cell Biochem 136, 139–144 (1994). https://doi.org/10.1007/BF00926074

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  • DOI: https://doi.org/10.1007/BF00926074

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