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Inputs from the olfactory bulb and olfactory cortex to the entorhinal cortex in the cat

I. Anatomical observations

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Summary

The spatial organization and laminar distribution of projections from the olfactory bulb and the anterior (PPCa) and posterior (PPCp) divisions of the prepiriform cortex to the entorhinal cortex were studied with anterograde (3H-leucine) and retrograde (WGA-HRP) tracing techniques. After 3H-leucine injections into the olfactory bulb transported labeling was seen over the lateral entorhinal area, except its most medial part, and over the rostral part of the medial entorhinal area. The labeling covers exclusively layer Ia. The lateral and medial entorhinal areas are also reached by fibers from the prepiriform cortex. The projection to the medial entorhinal area has not been described previously. Following injections of 3H-leucine into the PPCa transported labeling is present over the entire expanse of the entorhinal cortex and is located over layer Ib with the greatest density in its superficial part. Injections of 3H-leucine into the PPCp give rise to transported labeling over much of the entorhinal cortex. No labeling was found over the most medial parts of the medial subdivision (VMEA) of the lateral entorhinal area and the medial entorhinal area. Labeling occupies layer Ib, especially its middle part, and layers II and III. Both PPCa and PPCp appear to project most heavily to the dorsal (DLEA) and ventral (VLEA) subdivisions of the lateral entorhinal area. From the retrograde experiments it can be inferred that cells of layers II and III of the PPCa project predominantly to the DLEA, whereas those of the PPCp project predominantly to the VLEA. The MEA receives its heaviest projection from layer II of both PPCa and PPCp. In control experiments with 3H-leucine injections into the endopiriform nucleus it was found that this nucleus projects to the entire expanse of the entorhinal cortex. The fibers distribute to all layers with the exception of layer Ia.

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Abbreviations

AI:

agranular insular cortex

AL:

lateral nucleus of the amygdala

BL:

basolateral nucleus of the amygdala

BM:

basomedial nucleus of the amygdala

C:

claustrum

CoA:

cortical nucleus of the amygdala

DLEA:

dorsal division of the lateral entorhinal cortex

END:

endopiriform nucleus

H:

hippocampus

I:

granular insular cortex

lot:

lateral olfactory tractus

MCL:

mitral cell layer of the olfactory bulb

MEA:

medial entorhinal area

OB:

olfactory bulb

PPCa:

anterior part of the prepiriform nucleus

PPCp:

posterior part of the prepiriform nucleus

VLEA:

ventral division of the lateral entorhinal cortex

VMEA:

ventromedial division of the lateral entorhinal cortex

35:

area 35 of the perirhinal cortex

36:

area 36 of the perirhinal cortex

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Authors and Affiliations

  1. Department of Anatomy, Vrije University, v. d. Boechorststraat 7, NL-1081 BT, Amsterdam, The Netherlands

    P. Room, H. J. Groenewegen & A. H. M. Lohman

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  1. P. Room
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  2. H. J. Groenewegen
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  3. A. H. M. Lohman
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Room, P., Groenewegen, H.J. & Lohman, A.H.M. Inputs from the olfactory bulb and olfactory cortex to the entorhinal cortex in the cat. Exp Brain Res 56, 488–496 (1984). https://doi.org/10.1007/BF00237989

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  • DOI: https://doi.org/10.1007/BF00237989

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