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Bone formation in organ cultures of bone marrow

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Summary

Bone formation in organ cultures of intact marrow fragments from mouse is described. Marrow explants were cultured on the top surface of a millipore filter at a gas-liquid interface. Observations with both light- and electron microscopes demonstrated the formation of a well-organised trabecular matrix lined with osteoblast-like cells. The tissue and cells were positive for alkaline-phosphatase activity. Large amounts of thick, well-banded collagen fibrils and matrix vesicles typical of those found in bone were present. The tissue became mineralised in the presence of 10 mM Na-β-glycerophosphate; in its absence a similar trabecular matrix developed but mineralisation did not take place.

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Authors and Affiliations

  1. The Gamaleya Institute for Epidemiology and Microbiology, Academy of Medical Sciences of the USSR, Moscow, USSR

    E. A. Luria, A. J. Friedenstein & S. A. Kuznetsow

  2. MRC Bone Research Laboratory, Nuffield Department of Orthopaedic Surgery, Nuffield Orthopaedic Centre, Oxford, England

    M. E. Owen

  3. Department of Anatomy, University of Oxford, South Parks Road, Oxford, England

    J. F. Morris

Authors
  1. E. A. Luria
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  2. M. E. Owen
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  3. A. J. Friedenstein
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  4. J. F. Morris
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  5. S. A. Kuznetsow
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Luria, E.A., Owen, M.E., Friedenstein, A.J. et al. Bone formation in organ cultures of bone marrow. Cell Tissue Res. 248, 449–454 (1987). https://doi.org/10.1007/BF00218212

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  • DOI: https://doi.org/10.1007/BF00218212

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