Abstract
The current view on peroxisomes has changed dramatically from being human cell oddities to vital organelles that host several key metabolic pathways. To fulfil over 50 different enzymatic functions, human peroxisomes host either unique peroxisomal proteins or dual-localized proteins. The identification and characterization of the complete peroxisomal proteome in humans is important for diagnosis and treatment of patients with peroxisomal disorders as well as for uncovering novel peroxisomal functions and regulatory modules. Hence, here we compiled a comprehensive list of mammalian peroxisomal and peroxisome-associated proteins by curating results of several quantitative and non-quantitative proteomic studies together with entries in the UniProtKB and Compartments knowledge channel databases. Our analysis gives a holistic view on the mammalian peroxisomal proteome and brings to light potential new peroxisomal and peroxisome-associated proteins. We believe that this dataset, represents a valuable surrogate map of the human peroxisomal proteome.
Eden Yifrach, Sven Fischer—contributed equally to this work.
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Abbreviations
- MS:
-
Mass spectrometry
- PTS1:
-
Peroxisomal targeting signal 1
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Acknowledgements
We wish to thank Ronald Wanders and Hans Waterham for helpful discussions and Christian D. Peikert for help with extracting data from public databases. This work was supported by an ERC CoG 646604 Peroxisystem grant, the Deutsche Forschungsgemeinschaft (SFB 1190, FOR 1905, RTG 2202) and the Excellence Initiative of the German Federal & State Governments (EXC 294 BIOSS). MS is an incumbent of the Dr. Gilbert Omenn and Martha Darling Professorial Chair in Molecular Genetics.
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Yifrach, E., Fischer, S., Oeljeklaus, S., Schuldiner, M., Zalckvar, E., Warscheid, B. (2018). Defining the Mammalian Peroxisomal Proteome. In: del RÃo, L., Schrader, M. (eds) Proteomics of Peroxisomes. Subcellular Biochemistry, vol 89. Springer, Singapore. https://doi.org/10.1007/978-981-13-2233-4_2
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