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Molecular detection, community structure and phylogeny of ericoid mycorrhizal fungi

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Diversity and Integration in Mycorrhizas

Part of the book series: Developments in Plant and Soil Sciences ((DPSS,volume 94))

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Abstract

Through traditional culturing and molecular characterization, we have determined that five putative species and 2 polyphyletic assemblages of fungi produce ericoid mycorrhizae in Gaultheria shallon, other Ericaceae and Epacridaceae. Using phylogenetic analysis of ITS2 sequences in GenBank, we have confirmed that most of these fungi occur in North America, Europe, and Australia. The low recovery rate of culturable ericoid mycorrhizal fungi from Gaultheria shallon may partly be explained by the fact that most mycorrhizal root segments contain an unculturable basidiomycete, revealed by direct amplification, cloning, and sequencing of LSU fungal DNA from root. Molecular characterization and phylogenetic analysis are powerful tools in revealing the geographic distribution and identity of ericoid mycorrhizal fungi.

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References

  • Bergen R, Perotto S, Girlanda M, Vidano G and Luppi A M 2000 Ericoid mycorrhizal fungi are common root associates of Mediterranean ectomycorrhizal plants (Quercus ilex). Molecular Ecol. 9, 1639–1649.

    Article  Google Scholar 

  • Bonfante-Fasolo P 1980 Occurrence of a basidiomycete in living cells of mycorrhizal hair roots of Calluna vulgaris. Trans. British Mycol. Soc. 75, 320–325.

    Google Scholar 

  • Chambers S M, Liu G and Cairney J W G 2000 ITS rDNA sequence comparison of ericoid mycorrhizal endophytes from Woollsia pungen.r. Mycol. Res. 104, 168–174.

    Article  CAS  Google Scholar 

  • Chambers S M, Williams P G, Seppelt R D and Cairney J W G 1999 Molecular identification of Hymenoscvphus sp. from rhizoids of the leafy liverwort Cephaloziella exiliflora in Australia and Antarctica. Mycol. Res. 103, 286–288.

    Article  CAS  Google Scholar 

  • Currah R, Niemi M and Huhtinen S 1999 Oidiodendron maius and Scytalidium vaccinii from the mycorrhizas of Ericaceae in northern Finland. Karstenia 39, 65–68.

    Google Scholar 

  • Daple Y 1989 Ericoid mycorrhizal fungi in the Myxotrichaceae and Gymnoascaceae. New Phytol. 113, 523–527.

    Article  Google Scholar 

  • Egger K N and Sigler L 1993 Relatedness of the ericoid endophytes Syctalidium vaccinii and Hymenoscyphus ericae inferred from analysis of ribosomal DNA. Mycologia 85, 219–230.

    Article  CAS  Google Scholar 

  • Gernandt D S and Stone J K 1999 Phylogenetic analysis of nuclear ribosomal DNA places the nematode parasite, Drechmeria coniospora, in Clavicipitaceae. Mycologia 91, 993–1000.

    Article  CAS  Google Scholar 

  • Hambleton S, Egger K N and Currah R S 1998 The genus Oidiodendron: Species delimitation and phylogenetic relationships based on nuclear ribosomal DNA analysis. Mycologia 90, 855–870.

    Article  Google Scholar 

  • Hambleton S, Huhtinen S and Currah R S Hyrnenoscyphus ericae: A new record from western Canada. Mycol. Res. 103, 1391–1397.

    Google Scholar 

  • Lee S B and Taylor J W 1990 Isolation of DNA from fungal mycelia and simple spores. In PCR Protocols: A Guide to Methods and Applications. Eds. M A Innis, D H Gelfand, J J Sninsky and T J White. pp 282–287. Academic Press, San Diego.

    Google Scholar 

  • McLean C B, Cunnington J H and Lawrie A C 1999 Molecular diversity within and between ericoid endophytes from the Ericaceae and Epacridaceae. New Phytol. 144, 351–358.

    Article  CAS  Google Scholar 

  • Monreal M, Berch S M and Berbee M 1999 Molecular diversity of ericoid mycorrhizal fungi. Can. J. Bot. 77, 1580–1594.

    Article  CAS  Google Scholar 

  • Monreal M A 1997 Molecular identification of ericoid mycorrhizal fungi. Ph.D. Thesis. University of British Columbia, Department of Soil Science,Vancouver, BC.

    Google Scholar 

  • Pearson V and Read D J 1973 The biology of mycorrhiza in the Ericaceae. I. The isolation of the endophyte and synthesis of mycorrhizas in aseptic culture. New Phytol. 72, 371–379.

    Article  Google Scholar 

  • Perotto S. Actis-Perino E, Perugini J and Bonfante P 1996 Molecular diversity of fungi from ericoid mycorrhizal roots. Molecular Ecol. 5, 123–131.

    Article  CAS  Google Scholar 

  • Prescott C E and Weetman G F 1994 Salat Cedar Hemlock Integrated Research Program: A Synthesis. Faculty of Forestry, University of British Columbia,Vancouver. 85 pp.

    Google Scholar 

  • Prescott C E 1996 Salal Cedar Hemlock Integrated Research Program. Research Update #1: December 1996. Faculty of Forestry, University of British Columbia, Vancouver, B.C.

    Google Scholar 

  • Read D J 1983 The biology of mycorrhiza in the Ericales. Can. J. Bot. 61, 985–1004.

    Article  CAS  Google Scholar 

  • Read D J 1996 The structure and function of the ericoid mycorrhizal root. Ann. Bot. 77, 365–374.

    Article  CAS  Google Scholar 

  • Sharpies J M, Chambers S M, Meharg A A and Cairney J W G 2000 Genetic diversity of root-associated fungal endophytes from Calluna vulgaris at contrasting field sites. New Phytol. 148, 153–162.

    Article  Google Scholar 

  • Spatafora J W and Blackwell M 1993 Molecular systematics of unitunicate perithecial ascomycetes: The ClavicipitalesHypocreales connection. Mycologia 85, 912–922.

    Article  CAS  Google Scholar 

  • Straker C J 1996 Ericoid mycorrhiza: Ecological and host specificity. Mycorrhiza 6, 215–225.

    Article  Google Scholar 

  • Strandberg M and Johansson M 1999 Uptake of nutrients in Calluna vulgaris seed plants grown with and without mycorrhiza. For. Ecol. Manage. 114, 129–135.

    Article  Google Scholar 

  • Ursic M and Peterson R L 1997 Morphological and anatomical characterization of ectomycorrhizas and ectendomycorrhizas on Pinus strobus seedlings in a southern Ontario nursery. Can. J. Bot. 75, 2057–2072.

    Article  Google Scholar 

  • Warcup J H 1988 Mycorrhizal associations of isolates of Sehacina vermifera. New Phytol. 110, 227–232.

    Article  Google Scholar 

  • Weetman G E, Fournier R, Barker J, Schnorbus-Panozzo E and Germain A 1989 Foliar analysis and response of fertilized chlorotic Sitka spruce plantations on salal-dominated cedar-hemlock cutovers on Vancouver Island. Can. J. For. Res. 19, 1501–1511.

    Article  Google Scholar 

  • Weiss M and Oberwinkler F 2001 Phylogenetic relationships in Auriculariales and related groups — hypotheses derived from nuclear ribosomal DNA sequences. Mycol. Res. 105, 403–415.

    Article  CAS  Google Scholar 

  • White T J, Bruns T, Lee S and Taylor J 1990 Amplification and direct sequencing of fungal ribosomal RNA genes for phylogenetics. In PCR Protocols: A Guide to Methods and Applications. Eds. M A Innis, D HGelfand, J J Sninsky and T J White. pp 315–322 Academic Press, San Diego.

    Google Scholar 

  • Xiao G 1994 The role of root associated fungi in the dominance of salai. Ph.D. Thesis. University of British Columbia, Department of Botany, Vancouver BC.

    Google Scholar 

  • Xiao G and Berch S M 1995 The ability of known ericoid mycorrhizal fungi to form mycorrhizae with Gaultheria shallop. Mycologia 87, 467–470.

    Article  Google Scholar 

  • Xiao G and Berch S M 1996 Diversity and abundance of ericoid mycorrhizal fungi of Gaultheria shallon on forest clearcuts. Can. J. Bot. 74, 337–346.

    Article  Google Scholar 

  • Yamasaki S H, Fyles J W, Egger K N and Titus B D 1998 The effect of Kahnia angustifdlia on the growth, nutrition, and ectomycorrhizal symbiont community of black spruce. For. Ecol. Manage. 105, 197–207.

    Article  Google Scholar 

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© 2002 Springer Science+Business Media Dordrecht

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Berch, S.M., Allen, T.R., Berbee, M.L. (2002). Molecular detection, community structure and phylogeny of ericoid mycorrhizal fungi. In: Smith, S.E., Smith, F.A. (eds) Diversity and Integration in Mycorrhizas. Developments in Plant and Soil Sciences, vol 94. Springer, Dordrecht. https://doi.org/10.1007/978-94-017-1284-2_6

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  • DOI: https://doi.org/10.1007/978-94-017-1284-2_6

  • Publisher Name: Springer, Dordrecht

  • Print ISBN: 978-90-481-5933-8

  • Online ISBN: 978-94-017-1284-2

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