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Up-scaling application of microbial carbonate precipitation: optimization of urease production using response surface methodology and injection modification

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Abstract

In order to reduce the cost of the microbial-induced carbonate precipitation, mixotrophic growth of Sporosarcina pasteurii was carried out at different yeast extract/sodium acetate concentrations and constant chemical oxygen demand for optimal production of urease enzyme. Optimization of cultivation conditions was also investigated using a 3-level central composite design approach based on the response surface methodology. A good agreement between predicted values of enzyme activity and experimental results was observed (R 2 value of 0.973). All three chosen independent variables had statistically great effects on the efficiency of urease activity. The maximum activity of 2.98 mM urea min−1 was achieved at yeast extract concentration of 5 g L−1, NH4 concentration of 9.69 g L−1, and incubation time of 60 h as the optimal conditions. Thereafter, a novel injection procedure as sequencing batch mode injection has been proposed for bacteria and cementation fluid injection at obtained optimal urease activity. After fourth injection of bacteria and cementation fluid, uniform CaCO3 distribution with unconfined compression strength of 795 kPa was obtained even for poorly graded sand. The presented experimental approach for optimizing urease activity and strength production in porous media can be used to design the treatment protocol for practical engineering applications.

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References

  • Achal V, Mukherjee A, Basu P, Reddy M (2009) Lactose mother liquor as an alternative nutrient source for microbial concrete production by Sporosarcina pasteurii. J Ind Microbiol Biotechnol 36:433–438

    Article  CAS  Google Scholar 

  • Aghaeinejad-Meybodi A, Ebadi A, Shafiei S, Khataee A, Rostampour M (2015) Degradation of antidepressant drug Fluoxetine in aqueous media by ozone/H2O2 system: process optimization using central composite design. Env Technol 36(12):1477–1488. doi:10.1080/09593330.2014.994041

    Article  CAS  Google Scholar 

  • Al Qabany A, Soga K, Santamarina C (2011) Factors affecting efficiency of microbially induced calcite precipitation. J Geotech Geoenviron Eng 138:992–1001

    Article  Google Scholar 

  • Alkhatib M, Mamun AA, Akbar I (2015) Application of response surface methodology (RSM) for optimization of color removal from POME by granular activated carbon. Int J Environ Sci Technol 12:1295–1302

    Article  CAS  Google Scholar 

  • Al-Thawadi S (2008) High strength in-situ biocementation of soil by calcite precipitating locally isolated ureolytic bacteria. Phd Thesis, Murdoch University

  • Al-Thawadi S, Cord-Ruwisch R, Bououdina M (2012) Consolidation of sand particles by nanoparticles of calcite after concentrating ureolytic bacteria in situ. Int J Green Nanotechnol 4:28–36

    Article  CAS  Google Scholar 

  • Bang S, Lippert J, Yerra U, Mulukutla S, Ramakrishnan V (2010) Microbial calcite, a bio-based smart nanomaterial in concrete remediation. Int J Smart Nano Mater 1:28–39

    Article  CAS  Google Scholar 

  • Barkouki T, Martinez B, Mortensen B, Weathers T, De Jong J, Ginn T, Spycher N, Smith R, Fujita Y (2011) Forward and inverse bio-geochemical modeling of microbially induced calcite precipitation in half-meter column experiments. Transp Porous Media 90:23–39

    Article  CAS  Google Scholar 

  • Cheng L, Cord-Ruwisch R (2012) In situ soil cementation with ureolytic bacteria by surface percolation. Ecol Eng 42:64–72

    Article  Google Scholar 

  • DeJong J, Martinez B, Mortensen B, Nelson D, Waller J, Weil M, Ginn T, Weathers T, Barkouki T, Fujita Y (2009) Upscaling of bio-mediated soil improvement. In: Proceedings of 17th international conference on soil mechanics and geotechnical engineering, 5–9 October 2009, Alexandria, Egypt. Millpress Science Publishers, Rotterdam, pp 2300–2303

  • DeJong JT, Mortensen BM, Martinez BC, Nelson DC (2010) Bio-mediated soil improvement. Ecol Eng 36:197–210

    Article  Google Scholar 

  • Din MM, Ponraj M, Van Loosdrecht M, Ujang Z, Chelliapan S, Zambare V (2014) Utilization of palm oil mill effluent for polyhydroxyalkanoate production and nutrient removal using statistical design. Int J Environ Sci Technol 11:671–684

    Article  Google Scholar 

  • Fujita Y, Taylor JL, Wendt LM, Reed DW, Smith RW (2010) Evaluating the potential of native ureolytic microbes to remediate a 90Sr contaminated environment. Environ Sci Technol 44:7652–7658

    Article  CAS  Google Scholar 

  • Hammes F, Boon N, Clement G, de Villiers J, Siciliano SD, Verstraete W (2003) Molecular, biochemical and ecological characterisation of a bio-catalytic calcification reactor. Appl Microbiol Biotechnol 62:191–201

    Article  CAS  Google Scholar 

  • Harkes MP, Van Paassen LA, Booster JL, Whiffin VS, van Loosdrecht M (2010) Fixation and distribution of bacterial activity in sand to induce carbonate precipitation for ground reinforcement. Ecol Eng 36:112–117

    Article  Google Scholar 

  • Kumar NM, Ramasamy R, Manonmani H (2013) Production and optimization of l-asparaginase from Cladosporium sp. using agricultural residues in solid state fermentation. Ind Crops Prod 43:150–158

    Article  Google Scholar 

  • Le Metayer-Levrel G, Castanier S, Orial G, Loubiere J-F, Perthuisot J-P (1999) Applications of bacterial carbonatogenesis to the protection and regeneration of limestones in buildings and historic patrimony. Sed Geol 126:25–34

    Article  Google Scholar 

  • Maddipati P, Atiyeh HK, Bellmer DD, Huhnke RL (2011) Ethanol production from syngas by Clostridium strain P11 using corn steep liquor as a nutrient replacement to yeast extract. Bioresour Technol 102:6494–6501

    Article  CAS  Google Scholar 

  • Maleki M, Ebrahimi S, Asadzadeh F, Tabrizi ME (2016) Performance of microbial-induced carbonate precipitation on wind erosion control of sandy soil. Int J Environ Sci Technol 13:937–944

    Article  Google Scholar 

  • Mortensen B, Haber M, DeJong J, Caslake L, Nelson D (2011) Effects of environmental factors on microbial induced calcium carbonate precipitation. J Appl Microbiol 111:338–349

    Article  CAS  Google Scholar 

  • Mousavi SM, Niaei A, Salari D, Panahi PN, Samandari M (2013) Modelling and optimization of Mn/activate carbon nanocatalysts for NO reduction: comparison of RSM and ANN techniques. Environ Technol 34:1377–1384

    Article  CAS  Google Scholar 

  • Naik SS, Setty YP (2014) Optimization of parameters using response surface methodology and genetic algorithm for biological denitrification of wastewater. Int J Environ Sci Technol 11:823–830

    Article  Google Scholar 

  • Nemati M, Voordouw G (2003) Modification of porous media permeability, using calcium carbonate produced enzymatically in situ. Enzym Microbial Technol 33:635–642

    Article  CAS  Google Scholar 

  • Nemati M, Greene E, Voordouw G (2005) Permeability profile modification using bacterially formed calcium carbonate: comparison with enzymic option. Process Biochem 40:925–933

    Article  CAS  Google Scholar 

  • Stabnikov V, Naeimi M, Ivanov V, Chu J (2011) Formation of water-impermeable crust on sand surface using biocement. Cem Concr Res 41:1143–1149

    Article  CAS  Google Scholar 

  • Van Paassen L, Harkes M, Van Zwieten G, Van der Zon W, Van der Star W, Van Loosdrecht M (2009) Scale up of BioGrout: a biological ground reinforcement method. In: Proceedings of the 17th international conference on soil mechanics and geotechnical engineering, pp 2328–2333

  • Van Paassen LA, Ghose R, Van der Linden TJ, Van der Star WR, Van Loosdrecht MC (2010) Quantifying biomediated ground improvement by ureolysis: large-scale biogrout experiment. J Geotech Geoenvironl Eng 136:1721–1728

    Article  Google Scholar 

  • Van Tittelboom K, De Belie N, De Muynck W, Verstraete W (2010) Use of bacteria to repair cracks in concrete. Cem Concr Res 40:157–166

    Article  Google Scholar 

  • Washbourne C-L, Renforth P, Manning D (2012) Investigating carbonate formation in urban soils as a method for capture and storage of atmospheric carbon. Sci Total Environ 431:166–175

    Article  CAS  Google Scholar 

  • Whiffin VS (2004). Microbial CaCO3 precipitation for the production of biocement. PhD Thesis, Murdoch University

  • Whiffin VS, Van Paassen LA, Harkes MP (2007) Microbial carbonate precipitation as a soil improvement technique. Geomicrobiol J 24:417–423

    Article  CAS  Google Scholar 

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Acknowledgments

The authors thank Ahmad Sheikhbaglou (Biotechnology Research Center, Sahand University of Technology, Iran) for his valuable advice and help.

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Authors and Affiliations

  1. Biotechnology Research Centre, Faculty of Chemical Engineering, Sahand University of Technology, Tabriz, Iran

    M. M. Kakelar & S. Ebrahimi

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  1. M. M. Kakelar
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  2. S. Ebrahimi
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Correspondence to S. Ebrahimi.

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Kakelar, M.M., Ebrahimi, S. Up-scaling application of microbial carbonate precipitation: optimization of urease production using response surface methodology and injection modification. Int. J. Environ. Sci. Technol. 13, 2619–2628 (2016). https://doi.org/10.1007/s13762-016-1070-8

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  • DOI: https://doi.org/10.1007/s13762-016-1070-8

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