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Integrated landmark and outline-based morphometric methods efficiently distinguish species of Euglossa (Hymenoptera, Apidae, Euglossini)

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Abstract

Morphometric methods permit identification of insect species and are an aid for taxonomy. Quantitative wing traits were used to identify male euglossine bees. Landmark- and outline-based methods have been primarily used independently. Here, we combine the two methods using five Euglossa. Landmark-based methods correctly classified 84% and outline-based 77%, but an integrated analysis correctly classified 91% of samples. Some species presented significantly high reclassification percentages when only wing cell contour was considered, and correct identification of specimens with damaged wings was also obtained using this methodology.

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Acknowledgments

This work was supported by FAPESP (2011/0946-0 and 2011/07857-9 to T.M.F.) and CNPq (151947/2007-4 to T.M.F.)

Distinction efficace entre espèces d’ Euglossa (Hymenoptera, Apidae, Euglossini) grâce à l’utilisation combinée des méthodes morphométriques par points d’intérêt et par contours.

Euglossine / identification des espèces / morphométrie / aile / caractère quantitatif

Integration von landmarken- und umrissbasierten morphometrischen Methoden kann verschieden Arten von Euglossa (Hymenoptera, Apidae, Euglossini) effektiv unterscheiden.

Euglossine Bienen / Identifizierung von Arten / Morphometrie / Flügel / quantitative Merkmale

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Correspondence to Tiago Mauricio Francoy.

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Manuscript Editor: Marina Meixner

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Supplementary figure 1

Scatterplot of the discriminant analysis using partial warps and the centroid size from the five Euglossa species obtained in the landmark-based analysis. (JPEG 66 kb)

High-resolution image (TIFF 21 kb)

Supplementary figure 2

Scatterplot of the discriminant analysis using the 11 principal components obtained in the outline-based analysis of the second and third submarginal cells. (JPEG 72 kb)

High-resolution image (TIFF 23 kb)

Supplementary Table I

(PDF 68 kb)

Supplementary Table II

(PDF 73 kb)

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Francoy, T.M., de Faria Franco, F. & Roubik, D.W. Integrated landmark and outline-based morphometric methods efficiently distinguish species of Euglossa (Hymenoptera, Apidae, Euglossini). Apidologie 43, 609–617 (2012). https://doi.org/10.1007/s13592-012-0132-2

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  • DOI: https://doi.org/10.1007/s13592-012-0132-2

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