Abstract
The flowering in winter and wild wheats is induced by extended exposure to cold temperatures, a process called vernalization while spring wheats do not have any such requirement. In the present study, we analyzed 45 accessions of wild emmer wheat T. dicoccoides for the allelic composition of the vernalization genes at VRN-A1 and VRN-B1 loci. Three gene based markers for VRN-A1 and two for VRN-B1 locus were used. Thirty five T. dicoccoides accessions had only winter type allele vrn-A1 at VRN-A1 locus. One accession each had VRN-A1a and Vrn-A1d allele, and two accessions each showed the presence of VRN-A1b and Vrn-A1c, potent spring type alleles. Three accessions, however, had multiple alleles at VRN-A1 locus. At the VRN-B1 locus 35 accessions had winter type allele vrn-B1 and 10 accession had dominant Vrn-B1 allele. These T. dicoccoides accessions constitute a very useful resource for incorporation of alternative vernalization alleles in the cultivated wheat gene pool for developing better adaptive wheat varieties.
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References
Chen F, Gao M, Zhang J, Zuo A, Shang X, Cui D (2013) Molecular characterization of vernalization and response genes in bread wheat from the yellow and Huai Valley of China. MC Plant Biology 13:199
Dubcovsky J, Loukoianov A, Fu D, Valarik M, Sanchez A, Yan L (2006) Effect of photoperiod on regulation of wheat vernalization genes VRN1 and VRN2. Plant Mol Biol 60:469–480
Fu D, Szücs P, Yan L, Helguera M, Skinner JS, Zitzewitz J, Hayes PM, Dubcovsky J (2005) Large deletions within the VRN-1 first intron are associated with spring growth habit in barley and wheat. Mol Gen Genomics 273:54–65
Golovnina KA, Kondratenko EY, Blinvo AG, Goncharov NP (2010) Molecular characterization of vernalization loci VRN1 in wild and cultivated wheats. BMC Plant Biology 10:168
Nevo E (2001) Genetic resources of wild emmer, Triticum dicoccoides, for wheat improvement: in the third millennium. Isr J Plant Sci 49:77–91
Perrier X. and Jacquemoud-Collet J. P. 2006. DARwin software http://darwin.cirad.fr/darwin
Yan L, Loukoianov A, Tranquilli G, Helguera M, Fahima T, Dubcovsky J (2003) Positional cloning of the wheat vernalization gene VRN1. Proc Natl Acad Sci 100:6263–6268
Yan L, Helguera M, Kato K, Fukuyama S, Sherman J, Dubcovsky J (2004) Allelic variation at the VRN- promoter region in polyploid wheat. Theor Appl Genet 109:1677–1686
Acknowledgments
Financial assistance provided by Department of Biotechnology, Ministry of Science and Technology, Government of India, New Delhi in the form of various grants is gratefully acknowledged. The germplasm provided by the University of Haifa, Isreal is also gratefully acknowledged.
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Chhuneja, P., Arora, J.K., Kaur, P. et al. Characterization of wild emmer wheat Triticum dicoccoides germplasm for vernalization alleles. J. Plant Biochem. Biotechnol. 24, 249–253 (2015). https://doi.org/10.1007/s13562-014-0281-7
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DOI: https://doi.org/10.1007/s13562-014-0281-7