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Antibacterial, Antifungal and Mosquitocidal Efficacy of Copper Nanoparticles Synthesized from Entomopathogenic Nematode: Insect–Host Relationship of Bacteria in Secondary Metabolites of Morganella morganii sp. (PMA1)

  • Research Article-Biological Sciences
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Abstract

The present study deals with the use of cell-free supernatant of bacteria Morganella morganii for synthesizing copper nanoparticles and analysing its larvicidal activity on mosquito larvae. A colour change from blue to pickle green specifies the synthesis production of CuNPs. The nanoparticles were characterized using ultraviolet–visible spectrophotometry, scanning electron microscopy (SEM), X-ray diffraction (XRD) and Fourier transform infrared spectroscopy (FTIR). XRD pattern shows the major peaks, viz. (111), (200) and (220). SEM analysis shows that nanoparticles are in a spherical shape with a small percentage of elongated particles and size of about 13.5 ± 0.6 nm. A UV–Vis absorption peak was observed at 540 nm. FTIR analysis of nanoparticles exhibits functional groups such as aromatics, alkanes, ethers and alkyl halides. In EDaX (energy-dispersive X-ray) analysis, the peak signal confirms the presence of copper atoms that bound to the synthesized nanoparticles. The synthesized copper nanoparticles were tested for larvicidal efficacy at different time intervals. The above result shows that the copper nanoparticles produce increased toxicity in a time- and dose-dependent manner. An antibacterial and antifungal activity was tested against clinical pathogens, and the highest zone of inhibition was found to be in E. coli, B. subtilis, A. niger, M. anisopliae and Verticillium sp. This study shows that CuNPs possess a good antimicrobial and insecticidal activity which can be explored for commercial purpose.

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Acknowledgements

We would like to thank the Department of Biotechnology, Periyar University, Salem, Tamil Nadu, India, for providing the infrastructural facility for carrying out this research work. We would also acknowledge the instrument support from DST-FIST (SR/FIST/LSI-673/2016), Department of Biotechnology, Periyar University, Salem.

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Authors and Affiliations

  1. Molecular Entomology Laboratory, Department of Biotechnology, Periyar University, Salem, Tamil Nadu, 636 011, India

    Kandhasamy Lalitha, Kannan Nithya & Muthugounder Subramanian Shivakumar

  2. Department of Environmental Science, School of Life Sciences, Periyar University, Salem, Tamil Nadu, 636 011, India

    Dharman Kalaimurgan & Srinivasan Venkatesan

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  1. Kandhasamy Lalitha
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Correspondence to Muthugounder Subramanian Shivakumar.

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Lalitha, K., Kalaimurgan, D., Nithya, K. et al. Antibacterial, Antifungal and Mosquitocidal Efficacy of Copper Nanoparticles Synthesized from Entomopathogenic Nematode: Insect–Host Relationship of Bacteria in Secondary Metabolites of Morganella morganii sp. (PMA1). Arab J Sci Eng 45, 4489–4501 (2020). https://doi.org/10.1007/s13369-020-04487-6

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