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Genetic differentiation of Ganaspis brasiliensis (Hymenoptera: Figitidae) from East and Southeast Asia

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Abstract

Ganaspis brasiliensis (Ihering) (Hymenoptera: Figitidae: Eucoilinae) is a Drosophila parasitoid that has often been misidentified as G. xanthopoda (Ashmead) in recent studies. This study aims to clarify genetic differentiation of G. brasiliensis based on the nucleotide sequences of the mitochondrial cytochrome oxidase subunit 1 (CO1) gene and three nuclear DNA regions, the inter-transcribed spacers 1 and 2 (ITS1 and ITS2) and putative 60S ribosomal protein L37 (RpL37), as well as crossing experiments. Four lineages are recognized in individuals assigned as G. basiliensis by morphology, (1) individuals occurring in Japan and probably South Korea, (2) individuals from a small subtropical island of Japan, Iriomote-jima, (3) individuals from temperate lowlands of Japan and high altitude areas of Southeast Asia, and (4) individuals occurring widely in Asia, America, Hawaii and Africa. The first lineage is a specialist of Drosophila suzukii (Matsumura), a pest of fresh fruit, and also the fourth lineage has a capacity to parasitize this pest species. The first, third and fourth lineages occur sympatrically at least in Tokyo. The third and fourth lineages differed in mate choice and host use to some extent, but post-mating isolation between them was almost absent.

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Acknowledgments

We thank M. Kondo and M. B. Lankim who provided us with Ganaspis specimens from Kinabalu, and T. I. Kohyama, G. Kinoshita, and H. Suzuki for their advice and assistance in the molecular and phylogenetic analyses. This study was supported by Grant-in-Aids from the Japan Society for the Promortion of Science (No. 23370005) and also performed as a part of the Dropsa Project. The USDA does not endorse any commercial product mentioned in this research. USDA is an equal opportunity provider and employer.

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Authors and Affiliations

  1. Graduate School of Environmental Earth Science, Hokkaido University, Sapporo, Hokkaido, 060-0810, Japan

    Fumiaki Y. Nomano, Nazuki Kasuya & Akira Matsuura

  2. Zoology Division (Museum Zoologicum Bogoriense), Research Center for Biology - LIPI, Bogor, Cibinong, 16911, Indonesia

    Awit Suwito

  3. Tsurumaki 3-2-614, Tama, Tokyo, 206-0034, Japan

    Hideyuki Mitsui

  4. Systematic Entomology Laboratory, USDA, c/o National Museum of Natural History, Smithsonian Institution, Washington, DC, 20013, USA

    Matthew L. Buffington

  5. Hokkaido University Museum, Hokkaido University, Sapporo, Hokkaido, 060-0810, Japan

    Masahito T. Kimura

Authors
  1. Fumiaki Y. Nomano
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  2. Nazuki Kasuya
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  3. Akira Matsuura
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  4. Awit Suwito
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  5. Hideyuki Mitsui
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  6. Matthew L. Buffington
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  7. Masahito T. Kimura
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Correspondence to Masahito T. Kimura.

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Nomano, F.Y., Kasuya, N., Matsuura, A. et al. Genetic differentiation of Ganaspis brasiliensis (Hymenoptera: Figitidae) from East and Southeast Asia. Appl Entomol Zool 52, 429–437 (2017). https://doi.org/10.1007/s13355-017-0493-0

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  • DOI: https://doi.org/10.1007/s13355-017-0493-0

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