Skip to main content
Log in

Comparative study of dot enzyme immunoassay (Typhidot-M) and Widal test in the diagnosis of typhoid fever

  • Short Communication
  • Published:
Indian Pediatrics Aims and scope Submit manuscript

Abstract

We compared the sensitivity and specificity of Typhidot-M and Widal test with blood culture (gold standard) for diagnosing typhoid fever in 105 children aged 1–15 years admitted with clinical suspicion of typhoid fever. Of the 105 cases, blood culture was positive for S.typhi in 41 (39%) children, Widal test was positive in 48 (45.7%) and Typhidot-M was positive in 78 (74.3%) cases. Sensitivity and specificity of Typhidot-M was 92.6% and 37.5% while sensitivity and specificity of Widal test was 34.1% and 42.8%, respectively. In children with fever of less than 7 days duration, Typhidot-M was positive in 97%, compared to 24.2% by Widal test. Typhidot-M is a simple and sensitive test for early diagnosis of typhoid fever in children.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  1. Cleary TG, Salmonella. In: Behrman RE, Kliegman RM, Jenson HB, eds. Nelson Textbook of Pediatrics, 17th edition. Philadlphia: Saunders Publishers; 2004. p. 912–918.

    Google Scholar 

  2. Ivanoff B, Levine MM, Lambert PH. Vaccination against typhoid fever: present status. Bull World Health Organ 1994;72:957–971.

    CAS  PubMed  Google Scholar 

  3. Rajivi K, Gupta N, Shalini. Multidrug resistant typhoid fever. Indian J Pediatr 2007;74:39–42.

    Article  Google Scholar 

  4. Jesudason M, Esther E, Mathai E. Typhidot test to detect IgG and IgM antibodies in typhoid fever. Indian J Med Res 2002:116;70–72.

    PubMed  Google Scholar 

  5. Ananthanarayan R, Panikar CKJ. Text Book of Microbiology, 6th edition; Chennai: Orient Longman; 1999.

    Google Scholar 

  6. Nsutebu EF, Ndumbe PM, Shiro K. The increase in occurence of typhoid fever in Cameroon: Overdiagnosis due to misuse of the Widal test? Trans R Soc Trop Med Hyg 2002;96:64–67.

    Article  PubMed  Google Scholar 

  7. Olopoenia LA, King A. Widal agglutination test — 100 years later: still plagued by controversy. Postgrad Med J 2000; 76: 80–84.

    Article  CAS  PubMed  Google Scholar 

  8. Parry CM, Hoa NT, Diep TS, Wain J, Chinh NT, Vinh H, et al. Value of a single tube Widal test in diagnosis of typhoid fever in Vietnam. J Clin Microbiol 1999;37:2882–2886.

    CAS  PubMed  Google Scholar 

  9. Gopalakrishna V, Sekhar WY, Soo EH, Vinsent RA, Devi S. Typhoid fever in Kuala Lumpur and a comparative evaluation of two commercial diagnostic kits for the detection of antibodies to Salmonella typhi. Singapore Med J 2002;43:354–358.

    Google Scholar 

  10. Collantes E, Velmonte AM. The validity of the typhidot test in diagnosing typhoid fever among Filipinos. Phil J Microbiol Infect Dis 1997;26:61–63.

    Google Scholar 

  11. Bhutta ZA, Mansurali N. Rapid serologic diagnosis of pediatric typhoid fever in an endemic area: A prospective comparative evaluation of two dot enzyme immunoassays and the Widal test. Am J Trop Med Hyg 1999;61:654–657.

    CAS  PubMed  Google Scholar 

  12. Olsen SJ, Pruckler J, Bibb W, Thanh NTM, Trinh TM, Minh NT, et al. Evaluation of rapid diagnostic tests for typhoid fever. J Clin Microbiol 2004;42:1885–1889.

    Article  CAS  PubMed  Google Scholar 

  13. Jesudasson VM, Sivakumar S. Prospective evaluation of a rapid diagnostic test Typhidot for typhoid fever. Indian J Med Res 2006;123:513–516.

    Google Scholar 

  14. Shanta D, Dipika S, Byomkesh M, Bhaswati S, Deb AK, Jacqueline LD, et al. Evaluation of new generation serologic tests for the diagnosis of typhoid fever: data from a community based surveillance in Calcutta, India. Diagn Microbiol Infect Dis 2006; 56: 359–365.

    Article  Google Scholar 

Download references

Author information

Authors and Affiliations

Authors

Corresponding author

Correspondence to D. Narayanappa.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Narayanappa, D., Sripathi, R., JagdishKumar, K. et al. Comparative study of dot enzyme immunoassay (Typhidot-M) and Widal test in the diagnosis of typhoid fever. Indian Pediatr 47, 331–333 (2010). https://doi.org/10.1007/s13312-010-0062-x

Download citation

  • Received:

  • Revised:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s13312-010-0062-x

Key words

Navigation