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The chromosomal homology between dusky langur (Trachypithecus obscurus Ried, 1837) and human (Homo sapiens) revealed by chromosome painting

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Abstract

The presnt study provides illustated characterization of the chromosomal rearrangement of the male dusky langur (Trachypithecus obscurus Ried, 1837; TOB) using Chromosome Painting technique. Ear tissues specimen collection was kindly provided by Songkla Zoo, Songkla province, Thailand. The fibroblast cells were cultured and cryopreserved after that all 24 Human chromosome-specific probes (22 autosomes, X and Y) were hybridized in situ to the TOB mitotic cells and captured and analyzed by GENUS software. The results showed that the TOB chromosomes were highly rearranged compared to the 30 conserved regions of human chromosomes of different sizes. Majority, TOB chromosomes, such as TOB chromosomes 1, 2, 3, 4, 9, 11, 12, 13, 15, 17, 18, 20, 21, X and Y which were homologous to only one human chromosome, are conserved.. Some of those were constituted of regions being two TOB chromosomes that composed one of HSA homologous regions or one TOB chromosomes homologous to two human chromosomes. This is the first report of molecular cytogenetics on TOB using all 24 human chromosome-specific probes. The chromosome mappingis useful for a comparisonof Langur either in the same or other genus that had been reported to elucidate and clarify evolutionary relationship of primates shared by the same common ancestor.

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Acknowledgements

The financial support from Toxic Substances in Livestock and Aquatic Animals Research Group, Khon Kaen University is gratefully acknowledged.

Funding

Research Fund for Toxic Substances in Livestock and Aquatic Animals Research Group, Khon Kaen University.

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Authors and Affiliations

  1. Walai Rukhavej Botanical Research Institute, Mahasarakham University, Kantharawi-chai, Maha Sarakham, Thailand

    Weera Thongnetr

  2. Toxic Substances in Livestock and Aquatic Animals Research Group, Department of Biology, Faculty of Science, Khon Kaen University, Khon Kaen, 40002, Thailand

    Wiwat Sangpakdee & Alongklod Tanomtong

  3. Laboratory of Cellular and Molecular Evolution, Kunming Institute of Zoology, the Chinese Academy of Sciences, Kunming, Yunnan, China

    Wenhui Nie

  4. Department of Fundamental Science, Faculty of Science and Technology, Surindra Rajabhat University, Surin, 32000, Muang, Thailand

    Sayam Raso & Krit Pinthong

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  1. Weera Thongnetr
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Correspondence to Krit Pinthong.

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Thongnetr, W., Sangpakdee, W., Tanomtong, A. et al. The chromosomal homology between dusky langur (Trachypithecus obscurus Ried, 1837) and human (Homo sapiens) revealed by chromosome painting. Nucleus 65, 233–237 (2022). https://doi.org/10.1007/s13237-021-00381-0

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