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Entrapment characteristics of hydrosoluble vitamins loaded into chitosan and N,N,N-trimethyl chitosan nanoparticles

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Abstract

Nanoencapsulation is a process suitable for use in reducing degradation of instable components. In this study, chitosan and trimethyl chitosan with tripolyphosphate were used to nanoencapsulate vitamins C, B9, and B12. Analysis of the particle size showed that for a fix proportion of the polymer tripolyphosphate, the system showed a wide variation in size with the amount of added vitamins: e.g., for vitamin B9, the particle size varied from 150±5 nm to 809±150 nm. The zeta potential confirmed that trimethyl chitosan nanoparticles generally had a lower net positive charge (20 mV) than chitosan nanoparticles (40 mV). The encapsulation efficiency was found to be dependent on nanoparticle structure and vitamin solubility, with vitamin B9 the most efficiently encapsulated (approximately 40%). UV-Visible spectroscopy indicated different release profiles for vitamins C, B9, and B12 in a neutral PBS solution with release rates of 36%, 52%, and 16% after 2, 24, and 4 h, respectively. In conclusion the liberation was found to be slower in acidic media.

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Authors and Affiliations

  1. Embrapa Semiárido (CPATSA), BR 428 Km 152, Zona Rural, P.B. 23, CEP 56302-970, Petrolina, PE, Brazil

    Douglas de Britto

  2. Departamento de Física e Química, Faculdade de Engenharia de Ilha Solteira, UNESP — Univ. Estadual Paulista, 15385-000, Ilha Solteira, SP, Brazil

    Marcia R. de Moura & Fauze A. Aouada

  3. Laboratório Nacional de Nanotecnologia para o Agronegócio (LNNA), Embrapa Instrumentação (CNPDIA), R. XV de Novembro, 1452, CEP 13560-905, São Carlos, SP, Brazil

    Flávia G. Pinola, Odilio B. G. Assis & Luiz H. C. Mattoso

  4. Embrapa Pesca e Aquicultura, Quadra 104 Sul, Av. LO 1, 34, CEP 77020-020, Palmas, TO, Brazil

    Lícia M. Lundstedt

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  1. Douglas de Britto
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  2. Marcia R. de Moura
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  3. Fauze A. Aouada
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  4. Flávia G. Pinola
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  5. Lícia M. Lundstedt
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  6. Odilio B. G. Assis
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  7. Luiz H. C. Mattoso
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Correspondence to Douglas de Britto.

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de Britto, D., de Moura, M.R., Aouada, F.A. et al. Entrapment characteristics of hydrosoluble vitamins loaded into chitosan and N,N,N-trimethyl chitosan nanoparticles. Macromol. Res. 22, 1261–1267 (2014). https://doi.org/10.1007/s13233-014-2176-9

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  • DOI: https://doi.org/10.1007/s13233-014-2176-9

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