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Low-cost effective culture medium optimization for d-lactic acid production by Lactobacillus coryniformis subsp. torquens under oxygen-deprived condition

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Abstract

Lactic acid is considered a commodity and its production is boosted by the synthesis of polylactic acid. d-lactic acid (DLA) isomer offers greater flexibility and biodegradability and it can only be obtained in its pure form through fermentation. The lactate dehydrogenase is stereospecific for homofermentative production of DLA isomer in the metabolic pathway of Lactobacillus coryniformis subsp. torquens, with optical purity of ≥ 99.9% under oxygen-deprived condition. A simple culture medium that increases DLA production and reduces fermentation costs is fundamental for industrial applicability. A central composite rotatable design was used to evaluate significant components influencing the DLA production. Concentrations were adjusted using the Design-Expert 7.0 optimization tool with a desirability coefficient of 0.693 and the best concentrations of each component were determined. Finally, an assay in the bioreactor with the modified culture medium resulted in a product yield of 0.95 g/g, volumetric productivity of 0.85 g/L.h and 95% of efficiency.

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Acknowledgements

The authors gratefully acknowledge financial support by Leopoldo Américo Miguez de Mello Research and Development Center (Cenpes), Petrobras. We also would like to acknowledge the support of the Laboratories of Bioprocess Development (Ladebio), from the school of chemistry of Federal University of Rio de Janeiro, UFRJ.

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Authors and Affiliations

  1. Laboratory of Biocorrosion, Biodegradation and Biosynthesis, School of Chemistry, Federal University of Rio de Janeiro, Av. Horacio Macedo, 2030, Bloco E, Sala E-109, CT, Cidade Universitária - Ilha do Fundao, Rio de Janeiro, RJ, CEP: 21949-900, Brazil

    Lizeth Jaramillo & Diogo Dias

  2. Nucleus of Ecology and Socio-Environmental Development of Macaé, NUPEM, Federal University of Rio de Janeiro, Av. São José do Barreto, 764 -São José do Barreto, Macaé, RJ, CEP: 27965-045, Brazil

    Danielle Santos

  3. Department of Biochemical Engineering. School of Chemistry, Federal University of Rio de Janeiro, Av. Horacio Macedo, 2030, Bloco E, Sala E-203, CT, Cidade Universitária - Ilha do Fundao, Rio de Janeiro, RJ, CEP: 21949-900, Brazil

    Elcio Borges & Nei Pereira

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  1. Lizeth Jaramillo
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  2. Danielle Santos
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  3. Elcio Borges
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Correspondence to Lizeth Jaramillo.

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Jaramillo, L., Santos, D., Borges, E. et al. Low-cost effective culture medium optimization for d-lactic acid production by Lactobacillus coryniformis subsp. torquens under oxygen-deprived condition. Ann Microbiol 68, 547–555 (2018). https://doi.org/10.1007/s13213-018-1362-y

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  • DOI: https://doi.org/10.1007/s13213-018-1362-y

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