Abstract
A Gram-negative, rod-shaped, and non-motile bacterium, designated as isolate AD25T, was isolated from a flower of the blue trumpet vine (Thunbergia laurifolia) at Tong Pha Phum, Kanchanaburi, Thailand. Phylogenetic analyses of 16S rRNA gene, 16S-23S rRNA gene internal transcribed spacer (ITS) region, and groEL gene sequences showed that the isolate was quite remote and constituted a cluster independent from the type strains of other Acetobacter species. The isolate was closely related to Acetobacter cibinongensis, one of the closest relatives, with 98.3 % 16S rRNA gene sequence similarity. The DNA G -+- C content of the isolate was 51.4 mol%. The isolate grew intensely on 10 % ethanol with 1.5 % D-glucose in the presence of 0.3 % peptone and 0.3 % yeast extract, and grew weakly on 3.0 % D-glucose in the presence of 0.1 % ammonium sulfate as the sole source of nitrogen. The isolate produced only D-gluconic acid from D-glucose. Based on physiological, biochemical, and genotypic differences between the isolate and the type strains of the validly named species, it is proposed that the isolate be classified as a novel species of Acetobacter, for which the name Acetobacter thailandicus sp. nov. is introduced. The type strain is isolate AD25T (= BCC 15839T = NBRC 103583T).
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Acknowledgments
This work was supported financially by the Strategic Scholarship/Fellowships Research Network from the Office of the Higher Education Commission, Ministry of Education (Grant no. 82/2549). Sincere thanks are also due to Mr. Richard James Goldrick, Department of Foreign Languages, Kasetsart University for English editing of this manuscript. A part of this work was carried out through collaboration of the Core to Core Program supported by the Japan Society for the Promotion of Science (JSPS) and the National Research Council of Thailand (NRCT).
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Pitiwittayakul, N., Yukphan, P., Chaipitakchonlatarn, W. et al. Acetobacter thailandicus sp. nov., for a strain isolated in Thailand. Ann Microbiol 65, 1855–1863 (2015). https://doi.org/10.1007/s13213-014-1024-7
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DOI: https://doi.org/10.1007/s13213-014-1024-7