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Mangrove sediment, a new source of potential biosurfactant-producing bacteria

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Abstract

Biosurfactant-producing bacteria were isolated from mangrove sediment samples collected in the southern part of Thailand by an enrichment-culture technique in which lubricating oil was the sole carbon source. A total of 1,600 colonies were obtained, which were screened for biosurfactant production using the qualitative drop-collapsing test in a mineral salts medium containing 1% of different carbon sources (commercial sugar, glucose, molasses, and used lubricating oil). Ninety-five isolates were positive for biosurfactant production based on the results of this test, among which 20 could reduce the surface tension of the 48-h culture supernatant. The phylogenetic position of these 20 isolates was evaluated by 16S rRNA gene sequence analysis. The production of biosurfactants was determined for strains representative of eight different bacterial genera. Leucobacter komagatae 183, one of the newly isolated strains showing biosurfactant production, produced extracellular biosurfactants which reduced the surface tension of the culture supernatant from 72.0 to 32.0 m/Nm. Eighteen strains released extracellular emulsifiers able to stabilize the emulsion formed. Among these, the strains L. komagatae 183 and Ochrobactrum anthropi 11/6 exhibited emulsification activities comparable to those of synthetic surfactants. Overall, the new biosurfactant-producing strains isolated in this study display promising features for the future development and use in economically efficient industrial-scale biotechnological processes.

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Acknowledgments

The last author would like to thank the Office of the Higher Education Commission, Thailand for financial support for this work through a grant funded under the program Strategic Scholarships for Frontier Research Network for the Ph.D. Program Thai Doctoral degree. This work was also funded by the Faculty of Agro-Industry and Graduate School, Prince of Songkla University, and further supported by the TRF/BIOTEC Special Program for Biodiversity Research and Training grant BRT R651178.

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Authors and Affiliations

  1. Department of Food Science Technology, Faculty of Agriculture Technology, Phuket Rajabhat University, Phuket, 83000, Thailand

    Atipan Saimmai

  2. Institute of Plant Science and Resources, Okayama University, 2-20-1 Chuo, Kurashiki, 710-0046, Japan

    Akio Tani

  3. Department of Industrial Biotechnology, Faculty of Agro-Industry, Prince of Songkla University, Hat Yai, 90112, Thailand

    Vorasan Sobhon & Suppasil Maneerat

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  1. Atipan Saimmai
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  2. Akio Tani
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  4. Suppasil Maneerat
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Correspondence to Suppasil Maneerat.

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Saimmai, A., Tani, A., Sobhon, V. et al. Mangrove sediment, a new source of potential biosurfactant-producing bacteria. Ann Microbiol 62, 1669–1679 (2012). https://doi.org/10.1007/s13213-012-0424-9

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  • DOI: https://doi.org/10.1007/s13213-012-0424-9

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