Abstract
It is still difficult to directly detect low content of volatile organic compounds (VOCs) in water samples by gas chromatography (GC) because when water is the only solvent, it would result in the instability and poor repeatability of peak retention time and peak shape. The adverse effects of water on direct GC analysis of VOCs cannot be significantly reduced or eliminated by simply changing the detection condition of GC. However, it was found that the addition of methanol in samples to a certain final proportion, such as 50 or 75% (v/v), could greatly reduce or eliminate the adverse effects of water. By using 75% (v/v) methanol as a solvent, the standard curves of ethanol, acetic acid, acetone, and isopropanol with correlation coefficient (R2) over 0.99 were successfully plotted by gas chromatography-flame ionization detection (GC-FID) in a certain concentration range, respectively. The results showed that the retention time and peak shape stability of ethanol, acetic acid, acetone, and isopropanol in aqueous solution were greatly improved by the addition of methanol to final concertation of 75% (v/v). To verify the practical application potential of this method, the method was applied to the detection of components in isopropanol fermentation wastewater. The results showed that the method has well applicability and reliability. The key points in the application of this method were also summarized. This GC analysis method would have a wider and better application prospect in the detection of water-soluble organic matters.
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References
Baral NR, Slutzky L, Shah A, Ezeji TC, Cornish K, Christy A (2016) Acetone-butanol-ethanol fermentation of corn stover: current production methods, economic viability and commercial use. FEMS Microbiol Lett. https://doi.org/10.1093/femsle/fnw033
Chen JS, Hiu SF (1986) Acetone-butanol-isopropanol production by Clostridium beijerinckii. Biotechnol Lett 8(5):371–376
Chun HJ, Poklis JL, Poklis A, Wolf CE (2016) Development and validation of a method for alcohol analysis in brain tissue by headspace gas chromatography with flame ionization detector. J Anal Toxicol 40(8):653–658. https://doi.org/10.1093/jat/bkw075
Cui Y, He J, Yang KL, Zhou K (2020) Aerobic acetone-butanol-isopropanol (ABI) fermentation through a co-culture of Clostridium beijerinckii G117 and recombinant Bacillus subtilis 1A1. Metab Eng Commun 11:e00137. https://doi.org/10.1016/j.mec.2020.e00137
Drozd J, Novák J (1979) Headspace gas analysis by gas chromatography. J Chromatogr A 165(2):141–165. https://doi.org/10.1016/S0021-9673(00)90938-5
Fang Y, Sun G, Cong Q (2008) Effects of methanol on wettability of the non-smooth surface on butterfly wing. J Bionic Eng 5(2):127–133. https://doi.org/10.1016/S1672-6529(08)60016-5
Graffius GC, Jocher BM, Zewge D, Halsey HM, Lee G, Bernardoni F, Bu X, Hartman R, Regalado EL (2017) Generic gas chromatography-flame ionization detection method for quantitation of volatile amines in pharmaceutical drugs and synthetic intermediates. J Chromatogr A 1518:70–77. https://doi.org/10.1016/j.chroma.2017.08.048
Kang HI, Shin HS (2016) Determination of glutaraldehyde in water samples by headspace solid-phase microextraction and gas chromatography-mass spectrometry after derivatization with 2,2,2-trifluoroethylhydrazine. J Chromatogr A 1448:115–120 (S0021-9673(16)30489-7)
Martins ML, Donato FF, Prestes OD, Adaime MB, Zanella R (2013) Determination of pesticide residues and related compounds in water and industrial effluent by solid-phase extraction and gas chromatography coupled to triple quadrupole mass spectrometry. Anal Bioanal Chem 405(24):7697–7709. https://doi.org/10.1007/s00216-013-7235-0
Zhang SX, Peng R, Jiang R, Chai XS, Barnes DG (2018) A high-throughput headspace gas chromatographic technique for the determination of nitrite content in water samples. J Chromatogr A 1538:104–107. https://doi.org/10.1016/j.chroma.2018.01.026
Acknowledgements
This work was supported by a grant (BK20150417, BK20171266) from the Natural Science Foundation of Jiangsu Province of China; grants (21576110, 21706089) from the National Natural Science Foundation of China; a grant (17KJA180001) from Educational Commission of Jiangsu Province of China; grants (JPELBCPI2017003, JPELBCPI2015003) from Jiangsu Provincial Engineering Laboratory for Biomass Conversion and Process Integration Open Project.
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Zhou, J., Lu, X., Tian, B. et al. A gas chromatography-flame ionization detection method for direct and rapid determination of small molecule volatile organic compounds in aqueous phase. 3 Biotech 10, 520 (2020). https://doi.org/10.1007/s13205-020-02523-8
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DOI: https://doi.org/10.1007/s13205-020-02523-8