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Effect of Bacillus pumilus CCIBP-C5 on Musa–Pseudocercospora fijiensis interaction

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Abstract

The effect of antifungal activity of culture filtrate (CF) of Bacillus pumilus strain CCIBP-C5, an isolate from a phyllosphere of banana (Musa) leaves, was determined on Pseudocercospora fijiensis challenged banana plants. The CF was shown to decrease the fungal biomass and induce changes in banana plant. In this sense, at 70 days post inoculation (dpi), a lower infection index as well as a decrease in fungal biomass after 6 dpi was obtained in treated plants with respect to control ones. At the same time, changes in the activities of several enzymes related to plant defense responses, such as phenylalanine ammonia lyase, chitinases, β-1,3-glucanases and peroxidases were observed. These results indicate that B. pumilus CCIBP-C5 has a potential role for biological control of P. fijiensis possibly due to the production of antifungal metabolites.

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Acknowledgements

We thanks PhD. Roberto Vázquez and Miguel Tzec who helped develop the qPCR assays. The authors are also grateful to PhD Milady Mendoza and Mairenys Concepción for their comments and revisions of the manuscript. Partial financial support for this work was an output of a scholarship from the Food Security Center from the University of Hohenheim, which is part of the DAAD (German Academic Exchange Service) program “exceed” and in cooperation with the host PhD Blondy Canto Canché at the Centro de Investigación Científica de Yucatán (CICY), México.

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  1. Instituto de Biotecnología de las Plantas, Universidad Central “Marta Abreu” de Las Villas, Carretera a Camajuaní km 5.5, CP 54830, Santa Clara, Villa Clara, Cuba

    Mileidy Cruz-Martín, Mayra Acosta-Suárez, Eilyn Mena, Berkis Roque, Tatiana Pichardo & Yelenys Alvarado-Capó

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  1. Mileidy Cruz-Martín
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Cruz-Martín, M., Acosta-Suárez, M., Mena, E. et al. Effect of Bacillus pumilus CCIBP-C5 on Musa–Pseudocercospora fijiensis interaction. 3 Biotech 8, 122 (2018). https://doi.org/10.1007/s13205-018-1152-z

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