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Simple and fast multiplex PCR method for detection of species origin in meat products

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Abstract

Identification of animal species is one of the major concerns in food regulatory control and quality assurance system. Different approaches have been used for species identification in animal origin of feedstuff. This study aimed to develop a multiplex PCR approach to detect the origin of meat and meat products. Specific primers were designed based on the conserved region of mitochondrial Cytochrome C Oxidase subunit I (COX1) gene. This method could successfully distinguish the origin of the pig, camel, sheep, donkey, goat, cow, and chicken in one single reaction. Since PCR products derived from each species represent unique molecular weight, the amplified products could be identified by electrophoresis and analyzed based on their size. Due to the synchronized amplification of segments within a single PCR reaction, multiplex PCR is considered to be a simple, fast, and inexpensive technique that can be applied for identification of meat products in food industries. Nowadays, this technique has been considered as a practical method to identify the species origin, which could further applied for animal feedstuffs identification.

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Authors and Affiliations

  1. Human and Animal Cell Bank, Iranian Biological Resource Center (IBRC), ACECR, Tehran, Iran

    Mehrnaz Izadpanah, Nazanin Mohebali, Zahra Elyasi gorji, Parvaneh Farzaneh, Faezeh Vakhshiteh & seyed Abolhassan Shahzadeh Fazeli

  2. School of Advance Technologies in Medicine, Tehran University of Medical Science, Tehran, Iran

    Mehrnaz Izadpanah

  3. Department of Molecular and Cellular Biology, Faculty of Basic Sciences and Advanced Technologies in biology, University of Science and Culture, Tehran, Iran

    seyed Abolhassan Shahzadeh Fazeli

Authors
  1. Mehrnaz Izadpanah
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  2. Nazanin Mohebali
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  3. Zahra Elyasi gorji
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  4. Parvaneh Farzaneh
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  5. Faezeh Vakhshiteh
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  6. seyed Abolhassan Shahzadeh Fazeli
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Correspondence to Parvaneh Farzaneh or seyed Abolhassan Shahzadeh Fazeli.

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Izadpanah, M., Mohebali, N., Elyasi gorji, Z. et al. Simple and fast multiplex PCR method for detection of species origin in meat products. J Food Sci Technol 55, 698–703 (2018). https://doi.org/10.1007/s13197-017-2980-2

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  • DOI: https://doi.org/10.1007/s13197-017-2980-2

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