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Antidiabetic and antioxidant functionality associated with phenolic constituents from fruit parts of indigenous black jamun (Syzygium cumini L.) landraces

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Abstract

Fruit phenolics are important dietary antioxidant and antidiabetic constituents. The fruit parts (pulp, seed, seed coat, kernel) of underutilized indigenous six black jamun landraces (Syzygium cumini L.), found in Gir forest region of India and differed in their fruit size, shape and weight, are evaluated and correlated with antidiabetic, DPPH radical scavenging and phenolic constituents. The α-amylase inhibitors propose an efficient antidiabetic strategy and the levels of postprandial hyperglycemia were lowered by restraining starch breakdown. The sequential solvent systems with ascending polarity—petroleum ether, ethyl acetate, methanol and water were performed for soxhlet extraction by hot percolation method and extractive yield was found maximum with methanolic fruit part extracts of six landraces. The methanolic extracts of fruit parts also evidenced higher antidiabetic activity and hence utilized for further characterization. Among the six landraces, pulp and kernel of BJLR-6 (very small, oblong fruits) evidenced maximum 53.8 and 98.2% inhibition of α-amylase activity, respectively. The seed attained inhibitory activity mostly contributed by the kernel fraction. The inhibition of DPPH radical scavenging activity was positively correlated with phenol constituents. An HPLC–PDA technique was used to quantify the seven individual phenolics. The seed and kernel of BJLR-6 exhibited higher individual phenolics—gallic, catechin, ellagic, ferulic acids and quercetin, whereas pulp evidenced higher with gallic acid and catechin as α-amylase inhibitors. The IC50 value indicates concentration of fruit extracts exhibiting ≥50% inhibition on porcine pancreatic α-amylase (PPA) activity. The kernel fraction of BJLR6 evidenced lowest (8.3 µg ml−1) IC50 value followed by seed (12.9 µg ml−1), seed coat (50.8 µg ml−1) and pulp (270 µg ml−1). The seed and kernel of BJLR-6 inhibited PPA at much lower concentrations than standard acarbose (24.7 µg ml−1) considering good candidates for antidiabetic herbal formulations.

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Abbreviations

DPPH:

1,1-diphenyl-2-picrylhydrazyl

NIDDM:

Non-insulin dependent diabetes mellitus

WHO:

World health organization

BJLR:

Black jamun landraces

HPLC:

High-performance liquid chromatography

DNSA:

Di-nitro salicylic acid

PDA:

Photodiode array

2FCRD:

Two factor complete block randomized design

PPA:

Porcine pancreatic α-amylase

IC50 :

50% inhibition of enzyme activity

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Authors and Affiliations

  1. Department of Biotechnology, College of Agriculture, Junagadh Agricultural University, Junagadh, Gujarat, 362 001, India

    H. P. Gajera, Shila N. Gevariya, Darshna G. Hirpara, S. V. Patel & B. A. Golakiya

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  1. H. P. Gajera
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  2. Shila N. Gevariya
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  3. Darshna G. Hirpara
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  4. S. V. Patel
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  5. B. A. Golakiya
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Correspondence to H. P. Gajera.

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Gajera, H.P., Gevariya, S.N., Hirpara, D.G. et al. Antidiabetic and antioxidant functionality associated with phenolic constituents from fruit parts of indigenous black jamun (Syzygium cumini L.) landraces. J Food Sci Technol 54, 3180–3191 (2017). https://doi.org/10.1007/s13197-017-2756-8

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