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Aqueous extract of some indigenous medicinal plants inhibits glycation at multiple stages and protects erythrocytes from oxidative damage–an in vitro study

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Abstract

Azadirachta indica, Emblica officinalis, Syzygium cumini and Terminalia bellirica are common in Indian system of traditional medicine for the prevention of diabetes and its complications. The aim of the present study was to comprehensively and comparatively investigate the antiglycation potential of these plant extracts at multiple stages and their possible protective effect against glycated albumin mediated toxicity to erythrocytes. Antiglycation activities of these plant extracts was measured by co-incubation of plant extract with bovine serum albumin-fructose glycation model. The multistage glycation markers- fructosamines (early stage), protein carbonyls (intermediate stage) and AGEs (late stage) are investigated along with measurement of thiols and β aggregation of albumin using amyloid-specific dyes–Congo red and Th T. Protection of erythrocytes from glycated albumin induced toxicity by these plant extracts was assessed by measuring erythrocytes hemolysis, lipid peroxidation, reduced glutathione and intracellular antioxidant capacity. Total phenolics, reducing power and antioxidant activities of the plant extracts were also measured. In vitro glycation assays showed that plant extracts exerted site specific inhibitory effects at multiple stages, with T. bellirica showing maximum attenuation. In erythrocytes, along with the retardation of glycated albumin induced hemolysis and lipid-peroxidation, T. bellirica considerably maintained cellular antioxidant potential. Significant positive correlations were observed between erythrocyte protection parameters with total phenolics. These plant extracts especially T. bellirica prevents glycation induced albumin modifications and subsequent toxicity to erythrocytes which might offer additional protection against diabetic vascular complications.

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Abbreviations

ABTS:

2, 2′azino-bis (3-ethylbenzothizoline-6-sulphonic acid)

AGEs:

Advanced glycation end products

BHT:

Butylated hydroxyl toluene

BSA:

Bovine serum albumin

DNPH:

di-nitro phenyl hydrazine

DPPH:

2,2′-diphenyl-1-picrylhydrazyl

DTNB:

5, 5-Dithiobis- 2-nitrobenzoic acid

FC:

Folin–Ciocalteu

GAE:

Gallic acid equivalents

LPO:

Lipid peroxidation

MDA:

Malondialdehyde

OS:

Oxidative stress

RP:

Reducing power

Th. T:

Thioflavin T

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Acknowledgments

The financial assistance from Department of Science and Technology, New Delhi, India is gratefully acknowledged. The authors wish to record their sincere thanks to Principal G. D. Sharma and late Professor R. M. Kothari, for encouragement and helpful suggestions for the research work. We acknowledge Dr. S. Gaikwad (Biochemical Sciences Division, National Chemical Laboratory, and Pune) for help in spectroflurometric analysis. Part of this work was presented at the 43rd National Conference of Nutrition Society of India’s 50th meeting held at National Institute of Nutrition, Hyderabad, India during 11 to12 Nov. 2011.

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Authors and Affiliations

  1. Biochemical Sciences Division, Rajiv Gandhi Institute of IT and Biotechnology, Bharati Vidyapeeth University, Pune, 411046, India

    Rashmi S. Tupe, Neena M. Sankhe, Shamim A. Shaikh, Devyani V. Phatak, Juhi U. Parikh, Amrita A. Khaire & Nisha G. Kemse

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  1. Rashmi S. Tupe
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  2. Neena M. Sankhe
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  3. Shamim A. Shaikh
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  4. Devyani V. Phatak
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Correspondence to Rashmi S. Tupe.

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Tupe, R.S., Sankhe, N.M., Shaikh, S.A. et al. Aqueous extract of some indigenous medicinal plants inhibits glycation at multiple stages and protects erythrocytes from oxidative damage–an in vitro study. J Food Sci Technol 52, 1911–1923 (2015). https://doi.org/10.1007/s13197-013-1211-8

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