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MiR-144-3p: a novel tumor suppressor targeting MAPK6 in cervical cancer

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Abstract

Cervical cancer is the third most common gynecologic cancer in the world. Exploration of the molecular mechanism underlying cervical cancer pathogenesis will provide new insights into the development of novel therapies. In this study, we were aimed to characterize a novel miRNA in cervical cancer tumorigenesis. First, we measured the expressional change of miR-144-3p in clinical tissues and cancer cells. Second, we employed cell proliferation, cell migration, and invasion assays to understand its functional role in cervical cancer. Then, we confirmed in vitro findings in xenograft cancer model. Last, we mapped out a downstream target of miR-144-3p and validated its functional role in cancer cells. In the results, miR-144-3p was found significantly downregulated in cervical cancer cells and tissues. Over-expressing miR-144-3p suppressed cancer cells growth and metastasis. Consistent with in vitro results, over-expressing miR-144-3p led to tumor growth inhibition in vivo. Further on, MAPK6 was identified as an endogenous target of miR-144-3p in cervical cancer. Knocking down MAPK6 inhibited cervical cancer cells proliferation, migration, and invasion potential. Our investigation was the first time to report miR-144-3p as a tumor suppressive miRNA in cervical cancer. It inhibited tumor growth by targeting MAKP6. The newly identified signalling axis may serve as novel therapeutic targets to manage cervical cancer.

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Authors and Affiliations

  1. Heze Municipal Hospital, No. 2888, Caozhou Road, Heze, 274000, Shandong, China

    Jingli Wu, Yuying Zhao & Baohua Qiao

  2. The Second People’s Hospital of Caoxian County, Yuanqian Street, Qingguji Town, Caoxian County, Heze, 274400, Shandong, China

    Fenglian Li

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  1. Jingli Wu
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  2. Yuying Zhao
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  3. Fenglian Li
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  4. Baohua Qiao
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Correspondence to Baohua Qiao.

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Clinical evaluation of miR-144-3p expression in human cervical tumor tissues was approved by the Ethical Committee of Heze Municipal Hospital. The animal studies complied with Guide for the Care and Use of Laboratory Animals (8th edition, NIH) and was approved by the Animal Study Ethics Committee of Heze Municipal Hospital.

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Wu, J., Zhao, Y., Li, F. et al. MiR-144-3p: a novel tumor suppressor targeting MAPK6 in cervical cancer. J Physiol Biochem 75, 143–152 (2019). https://doi.org/10.1007/s13105-019-00681-9

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  • DOI: https://doi.org/10.1007/s13105-019-00681-9

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