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Isolation and characterisation of polymorphic genomic SSRs markers for the endangered tree Xanthoceras sorbifolium Bunge

  • Microsatellite Letters
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Abstract

Yellow-horn (Xanthoceras sorbifolium Bunge) is an endangered tree in northern China that has been devastated by humans during the past several decades. We have developed 84 microsatellite markers and tested them in eight trees sampled from four provinces. 38 loci were found to be polymorphic. The number of alleles per locus ranged from 2 to 8, and expected heterozygosity ranged from 0.117 to 0.855. 11 of 38 primers randomly selected also successfully amplified DNA from two population. These markers are essential for assessing the gene flow and mating system of Yellow-horn for conservation of this endangered species.

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References

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Acknowledgments

We thank Dr. Shiping Cheng, Zhiyong Zhang and Huabo Liu for assistance in dealing relevant SSR data and Ying Liu for assistance in experiment. This work was supported by the DUS program (2014018) and the Spark program of China (2013GA105004).

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Authors and Affiliations

  1. School of Nature Conservation, Beijing Forestry University, Qinghua East Road, Beijing, 100083, China

    Quanxin Bi & Wenbin Guan

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  1. Quanxin Bi
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  2. Wenbin Guan
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Correspondence to Wenbin Guan.

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Bi, Q., Guan, W. Isolation and characterisation of polymorphic genomic SSRs markers for the endangered tree Xanthoceras sorbifolium Bunge. Conservation Genet Resour 6, 895–898 (2014). https://doi.org/10.1007/s12686-014-0234-4

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  • DOI: https://doi.org/10.1007/s12686-014-0234-4

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