Skip to main content

Advertisement

SpringerLink
Log in

Polymorphic microsatellite markers for the endangered Hawaiian petrel (Pterodroma sandwichensis)

  • Technical Note
  • Published:
Conservation Genetics Resources Aims and scope Submit manuscript

Abstract

The endemic and endangered Hawaiian petrel (Pterodroma sandwichensis) breeds on four of the Hawaiian Islands, however the levels of gene flow between islands remain unclear. We describe 10 novel polymorphic microsatellite loci isolated from this species, and characterize 10 additional previously published primer sets in 40 individuals from the island of Maui. Loci exhibited between 3 and 14 alleles (mean 6.85), and observed heterozygosity ranged from 0.150 to 0.825 (mean 0.540). Four loci showed evidence for null alleles, although only one locus had significant deviation from Hardy–Weinberg equilibrium after Bonferonni correction. Two primer pairs showed significant gametic disequilibrium. The loci described here should provide a useful toolset for investigations of population connectivity.

This is a preview of subscription content, log in via an institution to check access.

Access this article

Log in via an institution

Price excludes VAT (USA)
Tax calculation will be finalised during checkout.

Instant access to the full article PDF.

Institutional subscriptions

References

  • Athens J, Kaschko M, James H (1991) Prehistoric bird hunters: high altitude resource exploitation on Hawai’i Island. Bishop Mus Occas Pap 31:63–84

    Google Scholar 

  • Bried J, Dubois M-P, Jouventin P, Santos R (2008) Eleven polymporhpic microsatellite markers in Cory’s shearwater, Calonectris diomedea, and cross-amplification on threatened Procellariiformes. Mol Ecol Resour 8:602–604

    Article  PubMed  CAS  Google Scholar 

  • Brooke M (2004) Albatrosses and petrels across the world. Oxford University Press, Oxford

    Google Scholar 

  • Brown RM, Jordan WC (2009) Characterization of polymorphic microsatellite loci from Round Island Petrels (Pterodroma arminjoniana) and their utility in other seabird species. J Ornithol 150:925–929

    Article  Google Scholar 

  • Burg TM (1999) Isolation and characterization of microsatellites in albatrosses. Mol Ecol 8:335–346

    Article  Google Scholar 

  • Dawson RJG, Gibbs HL, Hobson KA, Yezerinac SM (1997) Isolation of microsatellite DNA markers from a passerine bird, Dendroica petechia (the yellow warbler), and their use in population studies. Heredity 79:506–514

    Article  PubMed  CAS  Google Scholar 

  • Dearborn DC, Hailer F, Fleischer RC (2008) Microsatellite primers for relatedness and population structure in great frigatebirds (Pelecaniformes: Fregatidae). Mol Ecol Resour 8:1399–1401

    Article  PubMed  CAS  Google Scholar 

  • Friesen VL, González JA, Cruz-Delgado F (2006) Population genetic structure and conservation of the Galapagos petrel (Pterodroma phaeopygia). Conserv Genet 7(1):105–115. doi:10.1007/s10592-005-8704-6

    Article  Google Scholar 

  • Given AD, Mills JA, Baker AJ (2002) Isolation of polymorphic microsatellite loci from the red-billed gull (Larus novaehollandiae scopulinus) and amplification in related species. Mol Ecol Notes 2:416–418

    Article  CAS  Google Scholar 

  • Gonzalez EG, Genovart D, Oro D, Zardoya R, Juste J (2009) Polymorphic microsatellite markers for the critically endangered Balearic shearwater, Puffinus mauretanicus. Mol Ecol Resour 9:1044–1046

    Article  PubMed  CAS  Google Scholar 

  • Hamilton MB, Pincus EL, Di Fiore A, Fleischer RC (1999) Universal linker and ligation procedures for construction of genomic DNA libraries enriched for microsatellites. BioTechniques 27:500–507

    PubMed  CAS  Google Scholar 

  • Olson SL, James H (1982) Prodromus of the fossil avifauna of the Hawaiian Islands. Smithson Contrib Zool 365:1–59

    Google Scholar 

  • Rousset F (2008) GENEPOP’007: a complete re-implementation of the GENEPOP software for Windows and Linux. Mol Ecol Resour 8:103–106

    Article  PubMed  Google Scholar 

  • Rozen S, Skaletsky HJ (2000) Primer3 on the WWW for general users and biologist programmers. In: Krawetz S, Misener S (eds) Bioinformatics methods and protocols: methods in molecular biology. Humana Press, Totowa, NJ, pp 365–386

    Google Scholar 

  • Schuelke M (2000) An economic method for the fluorescent labeling of PCR fragments. Nat Biotechnol 18:233–234

    Article  PubMed  CAS  Google Scholar 

  • Simons TR, Hodges CN (1998) Dark-rumped Petrel (Pterodroma phaeopygia). In: Poole A (ed) The birds of North America online, vol 345. Cornell Lab of Ornithology, Ithaca

    Google Scholar 

  • Techow NMSM, O’Ryan C (2004) Characterization of microsatellite loci in White-chinned petrel (Procellaria aequinoctialis) and cross-amplification in six other procellariiform species. Mol Ecol Notes 4:33–35

    Article  CAS  Google Scholar 

  • van Oosterhout C, Hutchinson WF, Wills DPM, Shipley P (2004) Micro-Checker: software for identifying and correcting genotyping errors in microsatellite data. Mol Ecol Notes 4:535–538

    Article  Google Scholar 

  • Welch AJ, Yoshida AA, Fleischer RC (2011) Mitochondrial and nuclear DNA sequences reveal recent divergence in morphologically indistiguishable petrels. Mol Ecol. doi:10.1111/j.1365-294.X.2011.05008.X

Download references

Acknowledgments

We are grateful to Josh Adams, David Anderson, Fern Duvall, Nick Holmes, Jay Penniman, Helen James, Peggy Ostrom, Anne Wiley, Christina Gebhard, Megan Spitzer, Hawaii Volcanoes National Park, and the University of Washington Burke Museum for assistance obtaining samples. We also thank Frank Hailer, Nancy Rotzel, Michelle Haynie, Colleen Cotton, and the members of the CCEG lab group. Funding was provided by the Smithsonian’s Office of Fellowships, the Smithsonian Walcott Fund, and the NSF (grant DEB-0745604).

Author information

Authors and Affiliations

  1. Smithsonian Conservation Biology Institute, Center for Conservation and Evolutionary Genetics, National Zoological Park, P.O. Box 37012 MRC 5513, Washington, DC, 20013, USA

    Andreanna J. Welch & Robert C. Fleischer

  2. Behavior, Ecology, Evolution and Systematics Program, University of Maryland, College Park, MD, 20742, USA

    Andreanna J. Welch

Authors
  1. Andreanna J. Welch
    View author publications

    You can also search for this author in PubMed Google Scholar

  2. Robert C. Fleischer
    View author publications

    You can also search for this author in PubMed Google Scholar

Corresponding author

Correspondence to Andreanna J. Welch.

Rights and permissions

Reprints and permissions

About this article

Cite this article

Welch, A.J., Fleischer, R.C. Polymorphic microsatellite markers for the endangered Hawaiian petrel (Pterodroma sandwichensis). Conservation Genet Resour 3, 581–584 (2011). https://doi.org/10.1007/s12686-011-9409-4

Download citation

  • Received:

  • Accepted:

  • Published:

  • Issue Date:

  • DOI: https://doi.org/10.1007/s12686-011-9409-4

Keywords

Search

Navigation