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Genome Sequencing and Analysis of Bacillus pumilus ICVB403 Isolated from Acartia tonsa Copepod Eggs Revealed Surfactin and Bacteriocin Production: Insights on Anti-Staphylococcus Activity

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Abstract

Here we show that Bacillus pumilus ICVB403 recently isolated from copepod eggs is able to produce, after 48–72 h of growth in Landy medium, extracellular inhibitory compounds, which are active against Staphylococcus aureus ATCC 25923, methicillin-resistant S. aureus (MRSA) ATCC 43300, MRSA-S1, Staphylococcus epidermidis 11EMB, Staphylococcus warneri 27EMB, and Staphylococcus hominis 13EMB. Moreover, these extracellular inhibitory compound(s) were able to potentiate erythromycin against the aforementioned staphylococci. The minimum inhibitory concentration (MIC) of erythromycin was reduced from 32 μg/mL to 8 μg/mL for MRSA ATCC 43300 and MRSA SA-1 strains, and from 32–64 μg/mL to 4 μg/mL for S. epidermidis 11EMB and S. hominis 13EMB strains.

The genome sequencing and analysis of B. pumilus ICVB403 unveiled 3.666.195 nucleotides contained in 22 contigs with a G + C ratio of 42.0%, 3.826 coding sequences, and 73 RNAs. In silico analysis guided identification of two putative genes coding for synthesis of surfactin A, a lipopeptide with 7 amino acids, and for a circular bacteriocin belonging to the circularin A/uberolysin family, respectively.

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Acknowledgements

We would like to thank Dr. Matthieu Duban and Dr. Gabrielle Chataîgnée for their helpful assistance in the genome analysis and mass spectrometry analysis. We thank past and present members of the group of LOG COPEFISH team (SS) for their involvement in maintaining several cultures of copepods and algae and the Communauté d’Agglomération du Boulonnais (CAB) for supporting the implementation of a copepod-rearing pilot project (agreement Lille University-CAB).

Funding

This work is partly supported by CPER/FEDER Alibiotech grant (2016-2020) from la Région des Hauts-de-France. This work is a contribution to the project CPER 2014-2020 MARCO funded by the French government and the region Hauts-de-France.

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Authors and Affiliations

  1. Université de Lille, INRA, ISA, Université d’Artois, Université du Littoral-Côte d’Opale, EA 7394 – ICV-Institut Charles Viollette, Lille, France

    Mahammed Zidour, Yanath Belguesmia, Benoit Cudennec, Thierry Grard, Christophe Flahaut & Djamel Drider

  2. Université de Lille, CNRS, Université du Littoral Côte d’Opale, Laboratoire d’Océanologie et de Géosciences, UMR 8187 LOG, Wimereux, France

    Sami Souissi

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  1. Mahammed Zidour
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Correspondence to Yanath Belguesmia or Djamel Drider.

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Zidour, M., Belguesmia, Y., Cudennec, B. et al. Genome Sequencing and Analysis of Bacillus pumilus ICVB403 Isolated from Acartia tonsa Copepod Eggs Revealed Surfactin and Bacteriocin Production: Insights on Anti-Staphylococcus Activity. Probiotics & Antimicro. Prot. 11, 990–998 (2019). https://doi.org/10.1007/s12602-018-9461-4

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