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Genetic homogeneity revealed in micropropagated Bauhinia racemosa Lam. using gene targeted markers CBDP and SCoT

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Abstract

Two gene targeted markers i.e. CAAT box-derived polymorphism (CBDP) and start codon targeted (SCoT) polymorphism were applied to analyze the genetic stability of in vitro propagated plants of Bauhinia racemosa Lam. multiplied by enhanced axillary shoot proliferation of mature tree derived nodal explant. Nine randomly selected micropropagated plants of 1 year age were subjected to molecular analysis. The isolated genomic DNA samples were subjected to PCR amplification with a total of 61 primers (25 CBDP and 36 SCoT) out of which 39 primers (21 CBDP and 18 SCoT) produced scorable amplicons. A total of 97 and 88 clear, distinct and reproducible amplicons were produced by CBDP and SCoT primers, respectively. The monomorphic banding pattern obtained through all the tested primers corroborated the true to type nature of in vitro propagated plants of B. racemosa.

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Acknowledgements

Udit Sharma gratefully acknowledges University Grants Commission, New Delhi for providing financial assistance [F.17-78/2008 (SA-I)] in the form of Junior and Senior research fellowship.

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Authors and Affiliations

  1. Biotechnology Unit, Department of Botany (UGC-Centre of Advanced Study), Jai Narain Vyas University, New Campus, Jodhpur, Rajasthan, 342001, India

    Udit Sharma, Manoj K. Rai, N. S. Shekhawat & Vinod Kataria

  2. Department of Environmental Science, Indira Gandhi National Tribal University, Amarkantak, M.P., 484887, India

    Manoj K. Rai

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  1. Udit Sharma
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  2. Manoj K. Rai
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  3. N. S. Shekhawat
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  4. Vinod Kataria
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Correspondence to Vinod Kataria.

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Sharma, U., Rai, M.K., Shekhawat, N.S. et al. Genetic homogeneity revealed in micropropagated Bauhinia racemosa Lam. using gene targeted markers CBDP and SCoT. Physiol Mol Biol Plants 25, 581–588 (2019). https://doi.org/10.1007/s12298-018-00639-z

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  • DOI: https://doi.org/10.1007/s12298-018-00639-z

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