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Isolation, characterization, and evaluation of wild isolates of Lactobacillus reuteri from pig feces

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Abstract

Lactic acid bacteria (LAB) are a well-used probiotics for health improvements in both humans and animals. Despite of several benefits, non-host-specific LAB showed poor probiotics effects due to difficulty in colonization and competition with normal flora. Therefore, the feasibility of porcine LAB isolates was evaluated as a probiotics. Ten of 49 Lactobacillus spp. isolates harbored 2∼10 kb plasmid DNA. Seven strains were selected based on the safety test, such as hemolytic activity, ammonia, indole, and phenylalanine production. After safety test, five strains were selected again by several tests, such as epithelial adherence, antimicrobial activity, tolerance against acid, bile, heat, and cold-drying, and production of acid and hydrogen peroxide. Then, enzyme profiles (ZYM test) and antibiotics resistance were analyzed for further characterization. Five Lactobacillus reuteri isolates from pig feces were selected by safety and functional tests. The plasmid DNA which was able to develop vector system was detected in the isolates. Together with these approaches, pig-specific Lactobacillus spp. originated from pigs were selected. These strains may be useful tools to develop oral delivery system.

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  1. Deog Yong Lee

    Present address: Division of Enteric Bacterial Infections, Center for Infectious Diseases, National Institute of Health, Seoul, 122-701, Republic of Korea

Authors and Affiliations

  1. Department of Infectious Diseases, College of Veterinary Medicine, KRF Zoonotic Priority Research Institute and Brain Korea 21 Program for Veterinary Science, Seoul National University, Seoul, 151-742, Republic of Korea

    Deog Yong Lee, Yeon-Soo Seo, Nabin Rayamajhi, Mi Lan Kang, Su In Lee & Han Sang Yoo

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  1. Deog Yong Lee
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Correspondence to Han Sang Yoo.

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Lee, D.Y., Seo, YS., Rayamajhi, N. et al. Isolation, characterization, and evaluation of wild isolates of Lactobacillus reuteri from pig feces. J Microbiol. 47, 663–672 (2009). https://doi.org/10.1007/s12275-009-0124-8

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