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High-density single antibody electrochemical nanoarrays

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Abstract

The fabrication and electrochemical interrogation of very high density single-antibody nanoarrays is reported. Gold nanodots, 15 nm in diameter, arranged in large (cm2) square arrays with a pitch of 200 nm, are used as carriers for primary antibodies (immunoglobulin G (IgG)), further recognized by secondary redox-labeled detection antibodies. Ensemble scale interrogation of the antibody array by cyclic voltammetry, and nanoscale interrogation of individual nanodots by mediator tethered atomic force-scanning electrochemical microscopy (Mt/AFM-SECM), enable the occupancy of nanodots by single antibody molecules to be demonstrated. Experiments involving the competitive adsorption of antibodies of different species onto the nanodots evidence the possibility of using single-antibody nanoarrays for digital electrochemical immunoassays.

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Acknowledgements

This work has benefited from the facilities and expertise of the Mass Spectrometry platform of ICSN, Institut de Chimie des Substances Naturelle (Vincent Guérineau, CNRS, Gif-sur-Yvette, France, http://icsn.cnrs.fr).

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Authors and Affiliations

  1. Université Paris Cité, CNRS, Laboratoire d’Electrochimie Moléculaire, Paris, F-75013, France

    Khalil Chennit, Agnès Anne, Arnaud Chovin & Christophe Demaille

  2. Institute of Electronics, Microelectronics and Nanotechnology, CNRS, University of Lille, Avenue Poincaré, BP60069, Villeneuve d’Ascq, 59652, France

    Yannick Coffinier

  3. IIS, LIMMS/CNRS-IIS UMI2820, The University of Tokyo, 4-6-1 Komaba, Meguro-ku, Tokyo, 153-8505, Japan

    Shuo Li & Nicolas Clément

Authors
  1. Khalil Chennit
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  2. Yannick Coffinier
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  3. Shuo Li
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  4. Nicolas Clément
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  5. Agnès Anne
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  6. Arnaud Chovin
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  7. Christophe Demaille
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Correspondence to Nicolas Clément, Agnès Anne or Christophe Demaille.

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Chennit, K., Coffinier, Y., Li, S. et al. High-density single antibody electrochemical nanoarrays. Nano Res. 16, 5412–5418 (2023). https://doi.org/10.1007/s12274-022-5137-1

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  • DOI: https://doi.org/10.1007/s12274-022-5137-1

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