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Bacteria-assisted delivery and oxygen production of nano-enzyme for potent radioimmunotherapy of cancer

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Abstract

Tumor-targeting attenuated Salmonella could induce certain antitumor therapeutic effect through its proliferation characteristic and the consequent activated immune response, while host defense cells represented by neutrophils would trap and eliminate these invading bacteria via producing excess hydrogen peroxide (H2O2)-including reactive oxygen species in the bacteria-infected tumor, thereby impairing the efficacy of the bacteria treatment of tumor. Herein, we attempt to combine bacteria treatment and oxygen-dependent radioimmunotherapy of tumor through injection of neutrophil-targeted nano-catalase into the bacteria-treated mice for perfect tumor treatment outcome. Denatured albumin is used to coat catalase and deliver it to the neutrophils infiltrated in bacteria-infected tumor tissue. Taking advantage of the generating H2O2 by neutrophils, easily-diffused oxygen is produced and spread the whole tumor under the catalysis of nano-enzyme, leading to enhanced radiotherapy of hypoxic tumor cells. Moreover, the optimized tumor microenvironment, synergistically caused by potent immune-stimulation of bacteria, generating oxygen and tumor radiotherapy, would boost the antitumor immunity. This novel combination therapy strategy holds great promise to provide new ideas for future clinical cancer treatment.

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Acknowledgements

This work was partially supported by the National Natural Science Foundation of China (Nos. 32171382, U1932208 U2032134, and 31900986), the Project of State Key Laboratory of Radiation Medicine and Protection, Soochow University (No. GZK1202110), and the Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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  1. Jing Ni and Hailin Zhou contributed equally to this work.

Authors and Affiliations

  1. State Key Laboratory of Radiation Medicine and Protection, School of Radiation Medicine and Protection & School for Radiological and Interdisciplinary Sciences (RADX), Collaborative Innovation Center of Radiation Medicine of Jiangsu Higher Education Institutions, Soochow University, Suzhou, 215123, China

    Jing Ni, Hailin Zhou & Kai Yang

  2. School of Pharmacy, Jiangsu Key Laboratory of Inflammation and Molecular Drug Targets, Nantong University, Nantong, 226001, China

    Jingyu Gu, Xinpei Liu & Xuan Yi

  3. Dushu Lake Hospital Affiliated to Soochow University, Medical Center of Soochow University, Suzhou, 215123, China

    Jie Chen

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  1. Jing Ni
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  2. Hailin Zhou
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  3. Jingyu Gu
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  4. Xinpei Liu
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  5. Jie Chen
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  6. Xuan Yi
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Correspondence to Jie Chen, Xuan Yi or Kai Yang.

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Ni, J., Zhou, H., Gu, J. et al. Bacteria-assisted delivery and oxygen production of nano-enzyme for potent radioimmunotherapy of cancer. Nano Res. 15, 7355–7365 (2022). https://doi.org/10.1007/s12274-022-4369-4

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