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Proteomic Analysis of the Hippocampus in Mouse Models of Trigeminal Neuralgia and Inescapable Shock-Induced Depression

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Abstract

To investigate the behavioral and biomolecular similarity between neuralgia and depression, a trigeminal neuralgia (TN) mouse model was established by constriction of the infraorbital nerve (CION) to mimic clinical trigeminal neuropathic pain. A mouse learned helplessness (LH) model was developed to investigate inescapable foot-shock-induced psychiatric disorders like depression in humans. Mass spectrometry was used to assess changes in the biomolecules and signaling pathways in the hippocampus from TN or LH mice. TN mice developed not only significant mechanical allodynia but also depressive-like behaviors (mainly behavioral despair) at 2 weeks after CION, similar to LH mice. MS analysis demonstrated common and distinctive protein changes in the hippocampus between groups. Many protein function families (such as cell-to-cell signaling and interaction, and cell assembly and organization,) and signaling pathways (e.g., the Huntington’s disease pathway) were involved in chronic neuralgia and depression. Together, these results demonstrated that the LH and TN models both develop depressive-like behaviors, and revealed the involvement of many psychiatric disorder-related biomolecules/pathways in the pathogenesis of TN and LH.

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Acknowledgements

We thank Dr. Sheng-Yuan Yu at the Department of Neurology, Chinese PLA General Hospital for help with CION surgery. This work was supported by the National Natural Science Foundation of China (31421091, 81471130, 31371123 and 31420103903) and a Development Project of Shanghai Peak Disciplines Integrated Chinese and Western Medicine, China.

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  1. Qing-Huan Guo and Qing-He Tong have contributed equally to this work.

Authors and Affiliations

  1. Institutes of Brain Science, State Key Laboratory of Medical Neurobiology and Collaborative Innovation Center for Brain Science, Fudan University, Shanghai, 200032, China

    Qing-Huan Guo, Ning Lu, Hong Cao, Liu Yang & Yu-Qiu Zhang

  2. Shanghai Cancer Center and Institutes of Biomedical Sciences, Fudan University, Shanghai, 200032, China

    Qing-He Tong

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  1. Qing-Huan Guo
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  2. Qing-He Tong
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  3. Ning Lu
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Correspondence to Hong Cao or Yu-Qiu Zhang.

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Guo, QH., Tong, QH., Lu, N. et al. Proteomic Analysis of the Hippocampus in Mouse Models of Trigeminal Neuralgia and Inescapable Shock-Induced Depression. Neurosci. Bull. 34, 74–84 (2018). https://doi.org/10.1007/s12264-017-0131-4

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