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NRG-1 Stimulates Serum DJ-1 Increase in Breast Cancers

  • Original Article
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Pathology & Oncology Research

Abstract

To explore the relationship between the expression of DJ-1/HER3 and tumor grade in breast cancer, and investigate the effect of HER3 on NRG-1-mediated serum DJ-1 level in vivo. We analyze the expression level of DJ-1 and HER3 in 68 patients with different grades of breast cancer by immunostaining the tissue microarray. Besides, we investigated the serum DJ-1 level by ELISA. We found that the detectable DJ-1 protein expression is decreased, and the HER3 expression is increased in tumor tissue with the progression of breast cancer. There is a significant rise of DJ-1 in serum in vivo with the stimulation of NRG-1. Meanwhile, we found that HER3 knockdown abolishes NRG-1-induced serum DJ-1 increase and HER3 overexpress improves NRG-1-induced serum DJ-1 increase. This study provides a serum biomarker for breast cancer. The results showed that DJ-1 was associated with clinical stage of breast cancer, and NRG-1 increased the dissociation of HER3 and DJ-1, with promoting the level of DJ-1 in peripheral blood. It is suggested that the level of DJ-1 in peripheral blood may be conducive to assess the prognosis of patients with breast cancer and serum DJ-1 levels can serve as an indicator of therapeutic effectiveness for the development of HER3 targeting breast cancer antibody therapies.

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Acknowledgements

This project was sponsored by the National Natural Science Foundation of China (No. 81373398); a Project Funded by the Priority Academic Program Development of Jiangsu Higher Education Institutions (PAPD).

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Author notes

  1. Yuandong Wang, Yan Zhang and Qian Lu contributed equally to this work.

Authors and Affiliations

  1. Department of Radiotherapy, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu, 210029, People’s Republic of China

    Yuandong Wang, Qian Lu, Xinchen Sun & Shu Zhang

  2. Department of Anesthesiology, Union Hospital, Tongji Medical College, Huazhong University of Science and Technology, Wuhan, Hubei Province, 430022, China

    Yan Zhang

  3. School of Biotechnology and Biomolecular Sciences, University of New South Wales, Kensington, Sydney, NSW, 2052, Australia

    Yiming Wang

  4. Department of Radiotherapy, Clinical Research Center, The First Affiliated Hospital of Nanjing Medical University, 300 Guangzhou Road, Nanjing, Jiangsu, 210029, People’s Republic of China

    Shu Zhang

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  1. Yuandong Wang
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Correspondence to Shu Zhang.

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Wang, Y., Zhang, Y., Lu, Q. et al. NRG-1 Stimulates Serum DJ-1 Increase in Breast Cancers. Pathol. Oncol. Res. 25, 71–79 (2019). https://doi.org/10.1007/s12253-017-0326-z

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  • DOI: https://doi.org/10.1007/s12253-017-0326-z

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