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Standardized RT-PCR Conditions for Detection and Identification of Eleven Viruses of Potato and Potato spindle tuber viroid

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Abstract

Standardized RT-PCR procedures were developed and validated for detection of Alfalfa mosaic virus (AMV), Impatiens necrotic spot virus (INSV), Tobacco rattle virus (TRV), Tomato spotted wilt virus (TSWV), Potato leaf roll virus (PLRV), Potato mop top virus (PMTV), Potato virus A (PVA), Potato virus M (PVM), Potato virus S (PVS), Potato virus X (PVX), Potato virus Y (PVY), and Potato spindle tuber viroid (PSTVd). Under the same conditions the O, N, NTN, and N:O strains of PVY could be differentiated using previously published multiplex primers.

Resumen

Se desarrollaron y validaron procedimientos estandar RT-PCR para la detección de Virus Mosaico de la Alfalfa (AMV), Virus de la mancha necrótica de Impatiens (INSV), Virus Cascabelero del Tabaco (TRV), Virus de la Marchites manchada del jitomate (TSWV), Virus del Enrollamiento de la Hoja de Papa (PLRV), Virus de la proliferación apical de la Papa (PMTV), Virus A de la Papa (PVA), Virus M de la Papa (PVM), Virus S de la Papa (PVS), Virus X de la Papa (PVX), Virus Y de la Papa (PVY) y Viroide del Tubérculo Fusiforme de la Papa (PSTVd). Bajo las mismas condiciones se pudieron diferenciar las variantes O, N, NTN, y N:O del PVY usando iniciadores múltiples previamente publicados.

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Acknowledgements

This work was partially funded by the Washington State Potato Commission and grants from the National Potato Council. The use of trade, firm, or corporation names in this publication is for the information and convenience of the reader. Such use does not constitute an official endorsement or approval by the United States Department of Agriculture or the Agricultural Research Service of any product or service to the exclusion of others that may be suitable. USDA is an equal opportunity provider and employer.

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Authors and Affiliations

  1. USDA, Agricultural Research Service, 24106 North Bunn Road, Prosser, WA, 99350, USA

    James M. Crosslin & Launa L. Hamlin

  2. USDA-ARS Vegetable and Forage Crops Research Unit, Prosser, WA, 99350, USA

    James M. Crosslin & Launa L. Hamlin

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  1. James M. Crosslin
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  2. Launa L. Hamlin
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Correspondence to James M. Crosslin.

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Crosslin, J.M., Hamlin, L.L. Standardized RT-PCR Conditions for Detection and Identification of Eleven Viruses of Potato and Potato spindle tuber viroid . Am. J. Pot Res 88, 333–338 (2011). https://doi.org/10.1007/s12230-011-9198-z

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