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Comparative physiological and leaf proteome analysis between drought-tolerant chickpea Cicer reticulatum and drought-sensitive chickpea C. arietinum

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Abstract

Comparative physiological and proteomic analysis were performed to understand the stress responses of two chickpea species (C. reticulatum and C. arietinum) against drought. Our study revealed that drought stress reduced root length, leaf water content, and enhanced free proline content in both species. Effect of drought stress appeared to be greater in C. arietinum compared to C. reticulatum. A total of 24 differently expressed proteins were identified by using MALDI-TOF/TOF-MS/MS in response to drought. The proteins involved in photosynthesis and energy mechanisms were up-regulated in C. reticulatum and down-regulated in C. arietinum under drought. Our results suggest that the photosynthesis capacity of C. reticulatum is greater than that of C. arietinum under drought stress. Abundance of proline and sucrose biosynthesis related proteins, glutamine synthetase and cyctosolic fructose-bisphosphate aldolase, respectively, also increased in C. reticulatum under drought stress. The findings of this proteome analysis will help in understanding the mechanism of drought resistance in chickpea and may be also helpful in developing drought-resistant transgenic plants.

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Abbreviations

APX:

ascorbate peroxidase

COX:

cytochrome c oxidase

FBA:

fructose-bisphosphate aldolase

FNR:

ferredoxin-NADP reductase

GME:

GDP-mannose-epimerase

GS:

glutamine synthetase

G3PDH:

glyceraldehyde-3-phosphate dehydrogenase

IFR:

ısoflavone reductase

LHCB:

light-harvesting chlorophyll a/b-binding protein

LWP:

leaf water potential

OEE1:

oxygen evolving enhancer protein 1

OEE2:

oxygen evolving enhancer protein 2

PGK:

phosphoglycerate kinase

ROS:

reactive oxygen species

RWC:

relative water content

SBPase:

sedoheptulose-1,7-bisphosphatase

2D-PAGE:

two-dimensional polyacrylamide gel electrophoresis

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Acknowledgements

The authors would like to thank Prof. Dr. Cengiz Toker (Department of Field Crops, Faculty of Agriculture, Akdeniz University, Antalya, Turkey) for providing the plant material. The manuscript was linguistically supported by the Technology Transfer Office Academic Writing Center of Mersin University. This work was supported by the University of Mersin; Project number is BAP-FBE BB (SÇ) 2012-4 DR.

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Authors and Affiliations

  1. Vocational School of Mut, Mersin University, Mersin, Turkey

    Sertan Çevik

  2. Medical Biology Department/Dekart Proteomics Laboratory, Kocaeli University, Kocaeli, Turkey

    Gürler Akpinar & Murat Kasap

  3. Biology Department, Art and Science Faculty, Mersin University, Mersin, Turkey

    Aytunç Yildizli & Serpil Ünyayar

  4. Biomedical Engineering Department, Technology Faculty, Kocaeli, Turkey

    Kübra Karaosmanoğlu

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  1. Sertan Çevik
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Çevik, S., Akpinar, G., Yildizli, A. et al. Comparative physiological and leaf proteome analysis between drought-tolerant chickpea Cicer reticulatum and drought-sensitive chickpea C. arietinum. J Biosci 44, 20 (2019). https://doi.org/10.1007/s12038-018-9836-4

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