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Differential expression of speckled POZ protein, SPOP: Putative regulation by miR-145

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Abstract

The speckle POZ protein, SPOP, is an adaptor of the Cul3-based ubiquitination process, and has been implicated in the carcinogenesis process. Despite recent elucidation of biological functions, regulation of SPOP gene expression has not been reported. In this study, the mRNA levels of the mouse SPOP (mSPOP) gene were first shown to vary noticeably in different tissues. However, the SPOP protein was detected in high abundance only in Purkinje cells of the cerebellum and seminiferous tubule of the testis, echoing previous reports of involvement of ubiquitination in neuron cells and in spermatogenesis. In other mouse tissues and human cancer cell lines analysed, only low SPOP protein levels were detected. The 3′-untranslated regions of both the mSPOP and human SPOP transcripts harbor a conserved putative miR-145 binding site (BS). In some tissues and cell lines, miR-145 and SPOP protein levels were in an inverse relationship suggesting miR-145 regulation. Luciferase assays of deletion and point mutation constructs of the miR-145 BS, and miR-145 induction by serum starvation that resulted in reduced endogenous SPOP levels provided further evidence that miR-145 is likely involved in post-transcriptional regulation of SPOP expression in selected tissues, and possibly with the participation of other miRNA species.

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Abbreviations

BS:

binding site

MacroH2A1:

macrohistone H2A1

PDX-1:

pancreatic duodenal homeobox 1

PCIF1:

PDX-1 C-terminus-interacting factor 1

PIPKIIβ:

phosphatidylinositol phosphate kinase IIβ

Siah1A:

seven-in-absentia homolog 1A

SPOP:

speckled POZ protein

SRC-3:

steroid receptor co-activator-3

TNF:

tumour necrosis factor

TRAF:

TNF receptor-associated factor

Ube2i:

ubiquitin-conjugating enzyme 2i

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Acknowledgements

This work was supported in part by a grant (NSC-101-2313-B-034-003) from the National Science Council (Taiwan) to CJH, and by a University Tunku Abdul Rahman intramural grant 6200/C39 to KBC.

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Authors and Affiliations

  1. Department of Animal Science, Chinese Culture University, Yang Ming Shan, Taipei, Taiwan, 111

    Chiu-Jung Huang & Wan-Yi Lin

  2. Graduate Institute of Biotechnology, Chinese Culture University, Yang Ming Shan, Taipei, Taiwan, 111

    Chiu-Jung Huang & Wan-Yi Lin

  3. Graduate Program, Department of Biotechnology and Laboratory Science in Medicine, National Yang Ming University, Shipai, Taipei, Taiwan, 112

    Hsing-Yu Chen

  4. Department of Preclinical Sciences, Faculty of Medicine and Health Sciences, Universiti Tunku Abdul Rahman, Sg Long, Selangor, Malaysia

    Kong Bung Choo

  5. Centre for Stem Cell Research, Universiti Tunku Abdul Rahman, Sg Long, Selangor, Malaysia

    Kong Bung Choo

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  1. Chiu-Jung Huang
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  2. Hsing-Yu Chen
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  3. Wan-Yi Lin
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Corresponding author

Correspondence to Kong Bung Choo.

Additional information

Corresponding editor: Seyed E Hasnain

[Huang C-J, Chen H-Y, Lin W-Y and Choo KB 2014 Differential expression of speckled POZ protein, SPOP: Putative regulation by miR-145. J. Biosci. 39 1–13] DOI 10.1007/s12038-014-9432-1

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Huang, CJ., Chen, HY., Lin, WY. et al. Differential expression of speckled POZ protein, SPOP: Putative regulation by miR-145 . J Biosci 39, 401–413 (2014). https://doi.org/10.1007/s12038-014-9432-1

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