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Exogenous Neural Stem Cells Transplantation as a Potential Therapy for Photothrombotic Ischemia Stroke in Kunming Mice Model

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Abstract

Stroke is considered as the second leading cause of death worldwide. The survivors of stroke experience different levels of impairment in brain function resulting in debilitating disabilities. Current therapies for stroke are primarily palliative and may be effective in only a small population of stroke patients. In this study, we explore the transplantation of exogenous neural stem cells (NSCs) as the potential therapy for the photothrombotic ischemia stroke in a Kunming mice model. After stroke, mice receiving NSC transplantation demonstrated a better recovery of brain function during the neurobehavioral tests. Histology analysis of the brain samples from NSC transplanted mice demonstrated a reduction of brain damage caused by stroke. Moreover, immunofluorescence assay for biomarkers in brain sections confirmed that transplanted NSCs indeed differentiated to neurons and astrocytes, consistent with the improved brain function after stroke. Taken together, our data suggested that exogenous NSC transplantation could be a promising therapy for stroke.

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Acknowledgments

This study was supported by National Natural Science Foundation of Gansu, China (No. 1506RJZA222) and Lanzhou Science and Technology Bureau Project (2015-2-55).

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    Authors and Affiliations

    1. School of Life Sciences, Lanzhou University, No. 82 Cuiyingmen, Lanzhou, 730000, Gansu, China

      Boru Hou, Junning Ma, Xiumei Guo & Shengxiang Zhang

    2. Department of Neurosurgery, Lanzhou University Second Hospital, Lanzhou University, No. 82 Cuiyingmen, Lanzhou, 730030, Gansu, China

      Boru Hou, Junning Ma, Xiumei Guo, Furong Ju, Junwei Gao, Dengfeng Wang, Jixing Liu, Xiaohui Li & Haijun Ren

    Authors
    1. Boru Hou
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    2. Junning Ma
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    3. Xiumei Guo
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    4. Furong Ju
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    5. Junwei Gao
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    8. Xiaohui Li
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    9. Shengxiang Zhang
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    10. Haijun Ren
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    Corresponding authors

    Correspondence to Shengxiang Zhang or Haijun Ren.

    Ethics declarations

    The animal procedures were approved by Ethics Committee of Experimental Animals of Lanzhou University, in accordance with the “Guidelines for Experimental Animals” of the Ministry of Science and Technology (Beijing, China). All surgeries were performed according to the recommendations proposed by the Ethics Committee of Experimental Animals of Lanzhou University, and all efforts were made to minimize suffering

    Conflict of interest

    None

    Funding

    This study was supported by National Natural Science Foundation of Gansu, China (No. 1506RJZA222) and Lanzhou Science and Technology Bureau Project (2015-2-55).

    Additional information

    Boru Hou, Junning Ma and Xiumei Guo contributed equally to this work.

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    Cite this article

    Hou, B., Ma, J., Guo, X. et al. Exogenous Neural Stem Cells Transplantation as a Potential Therapy for Photothrombotic Ischemia Stroke in Kunming Mice Model. Mol Neurobiol 54, 1254–1262 (2017). https://doi.org/10.1007/s12035-016-9740-6

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    • DOI: https://doi.org/10.1007/s12035-016-9740-6

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