Abstract
The DNA molecular analyses together with ampelography, ampelometry, and biochemistry are essential for grapevine identification and investigation of genetic differences among the Vitis vinifera L. cultivars and clones. Ten Malvasia cultivars (i.e., Istrian Malvasia; M. delle Lipari; M. bianca di Candia; M. di Candia Aromatica; M. del Lazio; M. bianca lunga, also known as Malvasia del Chianti; M. nera di Brindisi/Lecce; M. di Casorzo; M. di Schierano, and M. nera di Bolzano) were analyzed using molecular approaches to study the genetic inter-varietal variability. Thirty Istrian Malvasia genotypes (i.e., 8 Italian clones, such as ISV 1, ISV F6, VCR 4, VCR 113, VCR 114, VCR 115, ERSA 120, ERSA 121, and 22 autochthonous grapevine accessions grown in Istrian Peninsula, Croatia) were investigated to evaluate the morphological and genetic intra-varietal variability. DNA analysis allowed discrimination of all Malvasia genotypes at molecular level using AFLP, SAMPL, and M-AFLP markers. Italian clones and autochthonous Croatian accessions of Istrian Malvasia were grouped according to their different geographic origins. These results showed the great genetic variability of Malvasia genotypes suggesting the need for the preservation of autochthonous grapevine biotypes found on different areas to approve the correct choice and selection of the grape multiplication materials.
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References
Mullis, L. G., Bouquet, A., & Williams, L. E. (1992). The biology of the grapevine. Cambridge, UK: Cambridge University Press.
Galet, P. (1979). A practical ampelography: Grapevine identification. Ithaca, New York: University Press.
Galet, P. (2000). Dictionnaire encyclopédique des cépages. Paris: Hachette.
Levadoux, L. (1956). Les populations sauvages et cultivees de Vitis vinifera L. Annales de l’Amélioration des Plantes, 6, 59–118.
Royo, J. B., Cabello, F., Miranda, S., Gogorcena, Y., González, J., Moreno, S., et al. (1997). The use of isoenzymes in characterization of grapevines (Vitis vinifera L.) Influence of the environment and time of sampling. Scientia Horticulturae, 69, 145–155.
Tessier, C., David, J., This, P., Boursiquot, J. M., & Charrier, A. (1999). Optimization of the choice of molecular markers for varietal identification in Vitis vinifera L. Theoretical and Applied Genetics, 89, 171–177.
Meneghetti, S., Costacurta, A., Frare, E., Da Rold, G., Migliaro, D., Morreale, G., et al. (2010). Clones identification and genetic characterization of Garnacha grapevine by means of different PCR-derived marker systems. Molecular Biotechnology. doi:10.1007/s12033-010-9365-3.
Loukas, M., Stavrakakis, M. N., & Krimbas, C. B. (1983). Inheritance of polymorphic isoenzymes in grape cultivars. Journal of Heredity, 74–3, 181–183.
Altube, H., Cabello, F., & Ortiz, J. M. (1991). Caracterización de variedades y portainjertos de vid mediante isoenzimas de los sarmientos. Vitis, 30, 203–212.
Bachmann, O., & Blaich, R. (1988). Isoeletric focusing of grapevine peroxidases as a tool for ampelography. Vitis, 27, 147–155.
Wolfe, W. H. (1976). Identification of Grape Varieties by Isozyme Banding Patterns. American Journal of Enology and Viticulture, 27–2, 68–73.
Pelsy, F., Schehrer, L., & Merdinoglu, D. (2003). Development of grapevine retrotransposon-based molecular markers (S-SAP). Acta Horticulturae, 603, 83–87.
Techera, G., Jubany, A., Ponce de León, S., Boido, I., Dellacassa, E., Carrau, E., et al. (2004). Molecular diversity (SSR) within clones of cv. Tannat (Vitis vinifera). Vitis, 43–4, 179–185.
Stavrakakis, M., & Loukas, M. (1983). The between and within grape cultivars genetic variation. Scientia Horticulturae, 19, 321–334.
Bachmann, K. (1994). Molecular markers in plant ecology. New Phytologist, 126, 403–418.
Regner, F., Wiedeck, E., & Stadlbauer, A. (2000). Differentiation and identification of White Riesling clones by genetic markers. Vitis, 39(3), 103–107.
Moreno, S., Gogorcena, Y., & Ortiz, J. M. (1995). The use of RAPD markers for identification of cultivated grapevine (Vitis vinifera L.). Scientia Horticulturae, 62–4, 237–243.
Böhm, A., & Zyprian, E. (1998). RAPD marker in grapevine (Vitis spp.) similar to plant retrotransposons. Plant Cell Reproduction, 17–5, 415–421.
Cervera, M. T., Cabezas, J. A., Sancha, J. C., Martínez de Toda, F., & Martínez-Zapater, J. M. (1998). Application of AFLPs to the characterization of grapevine Vitis vinifera L. genetic resources. A case of study with accessions from Rioja. Theoretical and Applied Genetics, 97–1(2), 51–59.
Fanizza, G., Chaabane, R., Ricciardi, L., & Resta, P. (2003). Analysis of a spontaneous mutant and selected clones of cv. Italia (Vitis vinifera) by AFLP markers. Vitis, 42–1, 27–30.
Blaich, R., Konradi, J., Rühl, E., & Forneck, A. (2007). Assessing genetic variation among Pinot noir (Vitis vinifera L.) clones with AFLP markers. American Journal of Enology and Viticulture, 58–4, 526–529.
Cretazzo, E., Meneghetti, S., De Andrés, M. T., Frare, E., Gaforio, L., & Cifre, J. (2010). Clone differentiation and varietal identification by means of SSR, AFLP, SAMPL and M-AFLP in order to assist the clonal selection of grapevine. The case of study of Manto Negro, Callet and Moll, autochthonous cultivars of Majorca. Annals of Applied Biology, 157–2, 213–227.
Wolf, T., Cabezas, J. A., & Martínez-Zapater, J. M. (2003). Genetic characterization of closely related rootstocks varieties based on AFLP and SAMPL markers. Acta Horticulturae, 603, 291–300.
Owens, C. L. (2003). SNP detection and genotyping in Vitis. Acta Horticulturae, 603, 139–140.
Labra, M., Imazio, S., Grassi, F., Rossoni, M., & Sala, F. (2004). Vine-1 retrotransposon-based sequence-specific amplified polymorphism for Vitis vinifera L genotyping. Plant Breeding, 123–2, 180–185.
Imazio, S., Labra, M., Grassi, F., Winfield, M., Bardini, M., & Scienza, A. (2002). Molecular tools (SSR, AFLP, MSAP) for clone identification: The case of the grapevine cultivar ‘Traminer’. Plant Breeding, 121–6, 531–535.
Pelsey, F., Schehrer, L., & Merdinoglu, D. (2002). Development of grapevine molecular markers based on retrotransposons. Acta Horticulturae, 603, 83–87.
D’Onofrio, C., De Lorenzis, G., Giordani, T., Natali, L., Scalabrelli, G., & Cavallini, A. (2009). Retrotransposon-based molecular markers in grapevine species and cultivars identification and phylogenetic analysis. Acta Horticulturae, 827, 45–52.
Albertini, E., Porceddu, A., Marconi, G., Barcaccia, G., Pallottini, L., & Falcinelli, M. (2003). Microsatellite-AFLP for genetic mapping of complex polyploids. Genome, 46, 824–832.
Meneghetti, S., Costacurta, A., & Calò, A. (2009). Evaluation of the intra-varietal variability for the clones identification (II). Oral communication N. CI-GENET 03.2009-07.1., O.I.V. 2009 experts genetic section. Paris, le 18 Mars 2009.
Crespan, M., Cabello, F., Giannetto, S., Ibáñez, J., Kontić, J. K., Maletić, E., et al. (2006). Malvasia delle Lipari, Malvasia di Sardegna, Greco di Gerace, Malvasia de Sitges and Malvasia dubrovačka—synonyms of an old and famous grape cultivar. Vitis, 45, 69–73.
Lacombe, T., Boursiquot, J. M., Laucou, V., Dechesne, F., Varès, D., & This, P. (2007). Relationships and genetic diversity within the accessions related to Malvasia held in the Domaine de Vassal Grape germplasm repository. American Journal of Enology and Viticulture, 58, 124–131.
Crespan, M., Coletta, A., Crupi, P., Giannetto, S., & Antonacci, D. (2008). Malvasia nera di Brindisi/Lecce’ grapevine cultivar (Vitis vinifera L.) originated from ‘Negroamaro’ and ‘Malvasia bianca lunga’. Vitis, 47–4, 205–212.
Calò, A., Scienza, A., & Costacurta, A. (2001). Vitigni d’Italia. Bologna, Italy: Calderini.
Calò, A., & Costacurta, A. (2005). Dei vitigni italici. Treviso, Italy: Matteo.
Poljuha, D., Sladonja, B., & Peršurić, D. (2006). Evaluation of intravarietal variability of cv. Istrian Malvasia by ampelographic parameters. Rivista di Viticoltura e Enologia, v59-2-3, 13–18.
Moretti, G. (2006). Vitigni e cloni d’Italia. Catalogo cloni 2006–2007. Vignevini, 12, 21–102.
O.I.V. (2006). 2nde edition de la liste des descripteurs OIV pour les varietes et especes de Vitis. Organisation Internationale de la vigne et du Vin, Paris.
Fabbris, L. (1997). Statistica multivariata, analisi esplorativa dei dati. Milano: McGraw-Hill.
This, P., Jung, A., Boccacci, P., Borrego, J., Botta, R., Costantini, L., et al. (2004). Development of a standard set of microsatellite reference alleles for identification of grape cultivars. Theoretical and Applied Genetics, 109, 1448–1458.
Crespan, M. (2003). The parentage of Muscat of Hamburg. Vitis, 42–4, 193–197.
Thomas, M. R., & Scott, N. S. (1993). Microsatellite repeats in grapevine reveal DNA polymorphisms when analysed as sequence tagged sites (STSs). Theoretical and Applied Genetics, 86, 985–990.
Bowers, J. E., Dangl, G. S., & Meredith, C. P. (1999). Development and characterization of additional microsatellite DNA markers for grape. American Journal of Enology and Viticulture, 53, 125–130.
Meneghetti, S., Calò, A., Costacurta, A., Frare, E., & Crespan, M. (2010). Valutazione della variabilità intra-varietale in vite ai fini dell’identificazione clonale—evaluation of the intra-varietal variability for the clones identification. Rivista Viticoltura e Enologia, 1-2–3-4, 93–103.
Meneghetti, S., Costacurta, A., Crespan, M., Maul, E., Hack, R., & Regner, F. (2009). Deepening inside the homonyms of Wildbacher by means of SSR markers. Vitis, 48–3, 123–129.
Barcaccia, G., Mazzucato, A., Albertini, E., Zethof, J., Gerats, A., Pezzotti, M., et al. (1998). Inheritance of parthenogenesis in Poa pratensis L.: Auxin test and AFLP linkage analyses support monogenic control. Theoretical and Applied Genetics, 96, 74–82.
Meneghetti, S., Barcaccia, G., Paiero, P., & Lucchin, M. (2007). Genetic characterization of Salix alba L. and Salix fragilis L. by means of different PCR-derived marker systems. Plant Biosystems, 141–143, 283–291.
Barcaccia, G., Meneghetti, S., Albertini, E., Triest, L., & Lucchin, M. (2003). Linkage mapping in tetraploid willows: Segregation of molecular markers and estimation of linkage phases support an allotetraploid structure for Salix alba × Salix fragilis interspecifc hybrids. Heredity, 90, 169–180.
Van Eijk, M., De Ruiter, M., Broekhof, J., & Peleman, J. (2001). Discovery and detection of polymorphic microsatellites by microsatellite-AFLP. In Plant and animal genome IX conference (p. 143).
Crespan, M. (2004). Evidence on the evolution of polymorphism of microsatellite markers in varieties of Vitis vinifera L. Theoretical and Applied Genetics, 108, 231–237.
Dangl, G. S., Mendum, M. L., Prins, B. H., Walzer, M. A., Meredith, C. P., & Simon, C. J. (2001). Simple sequence repeat analysis of a clonally propagated species: A tool for managing a grape germplasm collection. Genome, 44, 432–438.
Sneath, P. H. A., & Sokal, R. R. (1973). Numerical taxonomy (p. 513). San Francisco, CA: Freeman.
Lynch, M. (1990). The similarity index and DNA fingerprinting. Molecular Biology and Evolution, 7, 478–484.
Dice, L. R. (1945). Measures of the amount of ecologic association between species. Ecology, 26, 297–302.
Rohlf, F. J., & Sokal, R. R. (1981). Comparing numerical taxonomic studies. Systematic Zoology, 30, 459–490.
Tuimala, J. (2006). A primer to phylogenetic analysis using the PHYLIP package (5th ed., p. 55). Finland: The Author and CSC Espoo, Scientific Computing Ltd. http://www.ku.edu.np/biotech/bioinfodata/phylip2.pdf
Powell, W., Machray, G. C., & Provan, J. (1996). Polymorphism revealed by simple sequence repeats. Trends in Plant Science, 1, 215–222.
Meneghetti, S., Costacurta, A., Morreale, G., & Calò, A. (2011). Study of intravarietal genetic variability in grapevine cultivars by PCR-derived molecular markers and correlations with the geographic origin. Molecular Biothecnology. doi:10.1007/s12033-011-9403-9.
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This study was part of the “IDENTIVIT” project funded by Ministero delle Politiche Agricole Alimentari e Forestali (MiPAAF), Rome, Italy.
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Meneghetti, S., Poljuha, D., Frare, E. et al. Inter- and Intra-Varietal Genetic Variability in Malvasia Cultivars. Mol Biotechnol 50, 189–199 (2012). https://doi.org/10.1007/s12033-011-9423-5
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DOI: https://doi.org/10.1007/s12033-011-9423-5